Glucagon-d9 (trifluoroacetate salt)
目录号 : GC49342
An internal standard for the quantification of glucagon
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Glucagon-d9 is intended for use as an internal standard for the quantification of glucagon by GC- or LC-MS. Glucagon is a peptide hormone produced from proglucagon in pancreatic α cells that has a role in the regulation of glucose metabolism.1 Pulsatile application of glucagon to rat hepatocytes stimulates glucose production ex vivo.2 It also stimulates glucose output from perfused rat livers via increases in gluconeogenesis and glycogenolysis and decreases in glycolysis as well as increases plasma glucose levels in rats.1,3 Glucagon secretion is suppressed in response to food intake in normal rats and humans but is maintained or elevated in humans with type 2 diabetes.1
1.Jiang, G., and Zhang, B.B.Glucagon and regulation of glucose metabolismAm. J. Physiol. Endocrinol. Metab.284(4)E671-E678(2003) 2.Weigle, D.S., and Goodner, C.J.Evidence that the physiological pulse frequency of glucagon secretion optimizes glucose production by perifused rat hepatocytesEndocrinology118(4)1606-1613(1986) 3.Doi, Y., Iwai, M., Matsuura, B., et al.Glucagon attenuates the action of insulin on glucose output in the liver of the Goto-Kakizaki rat perfused in situPflugers Arch.442(4)537-541(2001)
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 0.2864 mL | 1.4319 mL | 2.8639 mL |
| 5 mM | 0.0573 mL | 0.2864 mL | 0.5728 mL |
| 10 mM | 0.0286 mL | 0.1432 mL | 0.2864 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。