Glutathione ethyl ester
(Synonyms: GSH-EE, GSH ethyl ester, GSH monoethyl ester) 目录号 : GC41293A cell-permeable derivative of glutathione
Cas No.:92614-59-0
Sample solution is provided at 25 µL, 10mM.
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Glutathione (GSH) serves as a nucleophilic co-substrate to glutathione transferase in the detoxification of xenobiotics and is an essential electron donor to glutathione peroxidases in the reduction of hydroperoxides.[1] [2][3] GSH ethyl ester is a cell-permeable derivative of GSH that undergoes hydrolysis by intracellular esterases to release GSH. [4] Effective transport of GSH ethyl ester has been used to protect cells against damage from radiation, oxidants, and various toxic compounds including heavy metals.[5] [6]
Reference：
[1]. Jakoby, W.B. The glutathione S-transferases: A group of multifunctional detoxification proteins. Advances in Enzymology and Related Areas of Molecular Biology 46, 383-414 (1978).
[2]. Baillie, T.A., and Slatter, J.G. Glutathione: A vehicle for the transport of chemically reactive metabolites in vivo. Acc. Chem. Res. 24(9), 264-270 (1991).
[3]. Bedia, C. Glutathione: Metabolism and function. (1976).
[4]. Wellner, V.P., Anderson, M.E., Puri, R.N., et al. Radioprotection by glutathione ester: Transport of glutathione ester into human lymphoid cells and fibroblasts. Proceedings of the National Academy of Sciences of the United States of America 81(15), 4732-4735 (1984).
[5]. Zeevalk, G.D., Manzino, L., Sonsalla, P.K., et al. Characterization of intracellular elevation of glutathione (GSH) with glutathione monoethyl ester and GSH in brain and neuronal cultures: Relevance to Parkinson’s disease. Experimental Neurology 203(2), 512-520 (2007).
[6]. Kelly-Aubert, M., Trudel, S., Fritsch, J., et al. GSH monoethyl ester rescues mitochondrial defects in cystic fibrosis models. Human Molecular Genetics 20(14), 2745-2759 (2011).
| Cas No. | 92614-59-0 | SDF | |
| 别名 | GSH-EE, GSH ethyl ester, GSH monoethyl ester | ||
| 化学名 | L-γ-glutamyl-L-cysteinyl-glycine, 3-ethyl ester | ||
| Canonical SMILES | O=C([C@H](CS)NC(CC[C@H](N)C(O)=O)=O)NCC(OCC)=O | ||
| 分子式 | C12H21N3O6S | 分子量 | 335.4 |
| 溶解度 | PBS (pH 7.2): 10 mg/ml,Water: 20 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.9815 mL | 14.9076 mL | 29.8151 mL |
| 5 mM | 0.5963 mL | 2.9815 mL | 5.963 mL |
| 10 mM | 0.2982 mL | 1.4908 mL | 2.9815 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet