Glyburide-d3
(Synonyms: 优降糖D3,Glyburide-d3) 目录号 : GC47406
An internal standard for the quantification of glyburide
Cas No.:1219803-02-7
Sample solution is provided at 25 µL, 10mM.
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Glyburide-d3 is intended for use as an internal standard for the quantification of glyburide by GC- or LC-MS. Glyburide is a sulfonylurea and an inhibitor of sulfonylurea receptor 1 (SUR1) linked to ATP-sensitive potassium channel Kir6.2 (IC50 = 4.3 nM).1 It binds to microsomes derived from RINm5F pancreatic β-cells (Kd = 0.3 nM) and inhibits 86Rb+ efflux from intact RINm5 cells with a half-maximal inhibition (K0.5) value of 0.06 nM.2 Glyburide (5 mg/kg) reduces blood glucose levels and increases the activity of hepatic glutathione-S-transferase (GST) and glucose-6-phosphate dehydrogenase (G6PDH) in a rat model of diabetes induced by streptozotocin .3 It inhibits ATP-induced increases in caspase-1 activation and IL-1β and IL-18 secretion in a concentration-dependent manner in LPS-primed bone marrow-derived macrophages (BMDMs).4 Glyburide (80 mg/ml) also reduces lesion growth in mice infected with L. mexicana.5 Formulations containing glyburide have been used in the treatment of type 2 diabetes.
1.Coghlan, M.J., Carroll, W.A., and Gopalakrishnan, M.Recent developments in the biology and medicinal chemistry of potassium channel modulators: Update from a decade of progressJ. Med. Chem.44(11)1627-1653(2001) 2.Schmid-Antomarchi, H., De Weille, J., Fosset, M., et al.The receptor for antidiabetic sulfonylureas controls the activity of the ATP-modulated K+ channel in insulin-secreting cellsThe Journal of Biological Chemisty262(33)15840-15844(1987) 3.Bugdayci, G., Altan, N., Sancak, B., et al.The effect of the sulfonylurea glyburide on glutathione-S-transferase and glucose-6-phosphate dehydrogenase in streptozotocin-induced diabetic rat liverActa. Diabetol.43(4)131-134(2006) 4.Lamkanfi, M., Mueller, J.L., Vitari, A.C., et al.Glyburide inhibits the Cryopyrin/Nalp3 inflammasomeJournal of Cell Biology187(1)61-70(2009) 5.Serrano-MartÍn, X., Payares, G., and Mendoza-LeÓn, A.Glibenclamide, a blocker of K+ATP channels, shows antileishmanial activity in experimental murine cutaneous leishmaniasisAntimicrobial Agents and Chemotherapy50(12)4214-4216(2006)
| Cas No. | 1219803-02-7 | SDF | |
| 别名 | 优降糖D3,Glyburide-d3 | ||
| Canonical SMILES | ClC1=CC(C(NCCC2=CC=C(S(NC(NC3CCCCC3)=O)(=O)=O)C=C2)=O)=C(OC([2H])([2H])[2H])C=C1 | ||
| 分子式 | C23H25ClD3N3O5S | 分子量 | 497 |
| 溶解度 | DMSO : 250 mg/mL (503.00 mM; Need ultrasonic); H2O : 0.1 mg/mL (0.20 mM; Need ultrasonic and warming) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.0121 mL | 10.0604 mL | 20.1207 mL |
| 5 mM | 0.4024 mL | 2.0121 mL | 4.0241 mL |
| 10 mM | 0.2012 mL | 1.006 mL | 2.0121 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet