Glycine-d5
(Synonyms: 甘氨酸-d5) 目录号 : GC49732
An internal standard for the quantification of glycine
Cas No.:4896-77-9
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Glycine-d5 is intended for use as an internal standard for the quantification of glycine by GC- or LC-MS. Glycine is a conditionally essential amino acid and the major inhibitory neurotransmitter in the spinal cord and brainstem.1,2 It is synthesized from threonine, choline, hydroxyproline, and serine via interorgan metabolism between the liver and kidneys.2 It can also be derived from dietary sources. Glycine is essential to protein synthesis and the conjugation of bile acids and is a precursor in glutathione, purine, heme, and serine synthesis.
1.Zafra, F., AragÓn, C., Olivares, L., et al.Glycine transporters are differentially expressed among CNS cellsJ. Neurosci.15(5 Pt 2)3952-3969(1995) 2.Wang, W., Wu, Z., Dai, Z., et al.Glycine metabolism in animals and humans: Implications for nutrition and healthAmino Acids45(3)463-477(2013)
| Cas No. | 4896-77-9 | SDF | Download SDF |
| 别名 | 甘氨酸-d5 | ||
| Canonical SMILES | [2H]N([2H])C([2H])([2H])C(O[2H])=O | ||
| 分子式 | C2D5NO2 | 分子量 | 80.1 |
| 溶解度 | Ethanol: 1 mg/ml,PBS (pH 7.2): 1 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 12.4844 mL | 62.422 mL | 124.8439 mL |
| 5 mM | 2.4969 mL | 12.4844 mL | 24.9688 mL |
| 10 mM | 1.2484 mL | 6.2422 mL | 12.4844 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
E.s.r. of spin-trapped radicals in aqueous solutions of deuterated amino acids and alcohols
Int J Radiat Biol Relat Stud Phys Chem Med 1980 Oct;38(4):395-415.PMID:6254899DOI:10.1080/09553008014551771
Selectively deuterated compounds can be utilized in spin-trapping to obtain conclusive evidence for the structures of the trapped radicals. Radicals formed from DL-alanine-2-d1, DL-alanine-3, 3, 3-d3, DL-alanine-d4, Glycine-d5, isopropyl-2-d1 alcohol, isopropyl-d7 alcohol and t-butyl-d9 alcohol were studied. The hydrogen abstraction, deamination and decarboxylation radicals generated from deuterated alanines were investigated by spin-trapping using t-nitrosobutane. Since nitroxides of the type XYZC-CH2-N(O)-tBu which contain an asymmetric centre next to the CH2 group exhibit unequal beta-proton splittings and temperature-dependent line width alternations, the effects of temperature on the e.s.r. spectra were studied. By these methods the structures of the trapped radicals were verified and the hyperfine coupling constants could be assigned without ambiguity. In gamma-irradiated polycrystalline amino acids, the observed deuterium exchange can be conveniently surveyed by dissolution in aqueous solutions containing t-nitrosobutane.