GnRH Associated Peptide (GAP) (1-13), human
(Synonyms: H2N-Asp-Ala-Glu-Asn-Leu-Ile-Asp-Ser-Phe-Gln-Glu-Ile-Val-OH ) 目录号 : GP10043
Inhibitor of prolactin secretion
Cas No.:100111-07-7
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Sequence: Asp-Ala-Glu-Asn-Leu-Ile-Asp-Ser-Phe-Gln-Glu-Ile-Val
The cloned complementary DNA sequence encoding the human gonadotropin-releasing hormone (GnRH) precursor protein was used to construct an expression vector for the bacterial synthesis of the 56-amino acid GnRH-associated peptide (GAP). GAP was found to be a potent inhibitor of prolactin secretion and to stimulate the release of gonadotropins. Active immunization with peptides corresponding to GAP sequences led to greatly increased prolactin secretion.
Gonadotropin-releasing hormone (GnRH) is known and named for its essential role in vertebrate reproduction. Release of this decapeptide from neurons in the hypothalamus controls pituitary gonadotropin levels that, in turn, regulate the gonadal state. The importance of GnRH is underscored by its widespread expression and conservation across vertebrate taxa. Also, GnRH-associated peptide (GAP) is co-secreted with GnRH into the hypophyseal portal bloodstream.
References:
1. KȢroly Nikolics,, Anthony J. Mason, ?va Sznyi, J. Ramachandran & Peter H. Seeburg, A prolactin-inhibiting factor within the precursor for human gonadotropin-releasing hormone, Nature 316, 511-517 (8 August 1985) doi:10.1038/316511a0
2. White SA, Kasten TL, Bond CT, Adelman JP, Fernald RD. Three gonadotropin-releasing hormone genes in one organism suggest novel roles for an ancient peptide. Proc Natl Acad SciUSA 92: 8363-8367, 1995.
3. Tianbao Song, Karoly Nikolics, Peter H. Seeburg, Paul C. Goldsmith, GnRH-prohormone-containing neurons in the primate brain: Immunostaining for the GnRH-Associated peptide, Peptides, Volume 8, Issue 2, March-April 1987, Pages 335-346
4. Ronchi E. ? Aoki C. ? Krey L.C. ? Pfaff D.W.
Immunocytochemical Study of GnRH and GnRH-Associated Peptide in Male Syrian Hamsters as a Function of Photoperiod and Gonadal Alterations, Neuroendocrinology 1992;55:134-145 (DOI:10.1159/000126108)
5. I.J. Clarke, J.T. Cummins, F.J. Karsch, P.H. Seeburg, K. Nikolics,GnRH-associated peptide (GAP) is cosecreted with GnRH into the hypophyseal portal blood of ovariectomized sheep, Biochemical and Biophysical Research Communications, Volume 143, Issue 2, 13 March 1987, Pages 665-671
| Cas No. | 100111-07-7 | SDF | |
| 别名 | H2N-Asp-Ala-Glu-Asn-Leu-Ile-Asp-Ser-Phe-Gln-Glu-Ile-Val-OH | ||
| 化学名 | GnRH Associated Peptide (GAP) (1-13), human | ||
| Canonical SMILES | CCC(C)C(C(=O)NC(C(C)C)C(=O)O)NC(=O)C(CCC(=O)O)NC(=O)C(CCC(=O)N)NC(=O)C(CC1=CC=CC=C1)NC(=O)C(CO)NC(=O)C(CC(=O)O)NC(=O)C(C(C)CC)NC(=O)C(CC(C)C)NC(=O)C(CC(=O)N)NC(=O)C(CCC(=O)O)NC(=O)C(C)NC(=O)C(CC(=O)O)N | ||
| 分子式 | C65H101N15O25 | 分子量 | 1492.58 |
| 溶解度 | ≥ 74.6mg/mL in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 0.67 mL | 3.3499 mL | 6.6998 mL |
| 5 mM | 0.134 mL | 0.67 mL | 1.34 mL |
| 10 mM | 0.067 mL | 0.335 mL | 0.67 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。