GPT Rat
目录号 : GP21722Glutamic-Pyruvate Transaminase Rat Recombinant
Sample solution is provided at 25 µL, 10mM.
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| Purity | Greaterthan 90.0% as determined by SDS-PAGE. | Source | E.coli. |
| Phycical Appearance | Sterile Filtered colorless solution. | Shipping Condition | Shipped with Ice Packs. |
| Synonyms | Alanine aminotransferase 1 (EC:2.6.1.2); ALT1; Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alaninetransaminase 1; Glutamic--pyruvic transaminase 1; Gpt; Aat1; Gpt1. | ||
| Amino Acid Sequence | MGSSHHHHHHSSGLVPRGSH MGSMASRVND QSQASRNGLKGKVLTLDTMN PCVRRVEYAV RGPIVQRALE LEQELRQGVK KPFTEVIRAN IGDAQAMGQR PITFFRQVLALCVYPNLLSS PDFPEDAKRR AERILQACGG HSLGAYSISS GIQPIREDVA QYIERRDGGI PADPNNIFLS TGASDAIVTMLKLLVSGEGR ARTGVLIPIP QYPLYSAALA ELDAVQVDYY LDEERAWALD IAELRRALCQ ARDRCCPRVLCVINPGNPTG QVQTRECIEA VIRFAFKEGL FLMADEVYQD NVYAEGSQFH SFKKVLMEMG PPYSTQQELASFHSVSKGYM GECGFRGGYV EVVNMDAEVQ KQMGKLMSVR LCPPVPGQAL MDMVVSPPTP SEPSFKQFQAERQEVLAELA AKAKLTEQVF NEAPGIRCNP VQGAMYSFPQ VQLPLKAVQR AQELGLAPDM FFCLCLLEETGICVVPGSGF GQQEGTYHFR MTILPPMEKL RLLLEKLSHF HAKFTHEYS. | ||
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. | ||
| Biological Activity | Specific activity is> 60units/mg, and is defined as the amount of enzyme that cleaves 1umole ofL-Alanine to L-Glutamate per minute at pH 7.5 at 37C. | ||
| Formulation | GPT protein solution (1mg/ml) containing PhosphateBuffered Saline pH 7.4 and 10% glycerol. | ||
GPT catalyzes the reversible transamination between alanine and 2-oxoglutarate to create pyruvate and glutamate. GPT has a crucial part in the intermediary metabolism of glucose and amino acids. GPT is broadly used as an indicator of liver reliability or hepatocellular destruction in clinical tests.
Specific activity is> 60units/mg, and is defined as the amount of enzyme that cleaves 1umole ofL-Alanine to L-Glutamate per minute at pH 7.5 at 37C.
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.