Gramicidin
(Synonyms: 短杆菌肽) 目录号 : GC32076
A polypeptide antibiotic mixture
Cas No.:1405-97-6
Sample solution is provided at 25 µL, 10mM.
Gramicidin is a polypeptide antibiotic mixture of gramicidin A , gramicidin B, and gramicidin C originally isolated from B. brevis.1 It is also a component of the antibiotic tyrothricin .2 Gramicidin is active against the Gram-positive bacterium S. aureus but not Gram-negative E. coli.3 It also protects mice from Pneumococcus infection in vivo when administered at a dose of 0.002 mg/animal. Gramicidin inhibits growth of bacteria by forming pores and channels in the cell wall, which increases its permeability to monovalent cations.1 Formulations containing gramicidin have been used as topical agents in the treatment of bacterial skin infections.
1.Wallace, B.A.Gramicidin channels and poresAnnu. Rev. Biophys. Biophys. Chem.19127-157(1990) 2.Brewer, G.A.GramicidinAnalytical profiles of drug substances8179-218(1979) 3.Dubos, R.J., and Hotchkiss, R.D.The production of bactericidal substances by aerobic sporulating bacilliJ. Exp. Med.73(5)629-640(1941)
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Cell experiment: |
Microbes and dispersions are mixed and interacted for one hour before being diluted up to 20,000-fold for plating of 0.1 mL of each in triplicate and incubation (24 hours for 37°C) for CFU counting. Cell survival (%), taken as the mean±standard deviation, is plotted against DODAB and/or Gramicidin concentration. As a control for cell viability in the absence of DODAB or DODAB/Gramicidin dispersions, a standard bacterial suspension is added to 0.264 M D-glucose solution, diluted, and spreaded on the agar plate[1]. |
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References: [1]. Ragioto DA, et al. Novel gramicidin formulations in cationic lipid as broad-spectrum microbicidal agents. Int J Nanomedicine. 2014 Jun 30;9:3183-92. |
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| Cas No. | 1405-97-6 | SDF | |
| 别名 | 短杆菌肽 | ||
| Canonical SMILES | [Gramicidin] | ||
| 分子式 | C99H140N20O17 | 分子量 | 1882.3 |
| 溶解度 | DMSO : ≥ 100 mg/mL (53.13 mM);Water : < 0.1 mg/mL (insoluble) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.5313 mL | 2.6563 mL | 5.3126 mL |
| 5 mM | 0.1063 mL | 0.5313 mL | 1.0625 mL |
| 10 mM | 0.0531 mL | 0.2656 mL | 0.5313 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet