Green CMFDA
(Synonyms: 5-氯甲基荧光素二乙酸酯,5-Chloromethylfluorescein diacetate) 目录号 : GC43783
绿色 CMFDA 作为一种非末端、非荧光探针，可以被活细胞常见的非特异性酯酶切割，产生荧光化合物荧光素，使用荧光显微镜可见。
Cas No.:136832-63-8
Sample solution is provided at 25 µL, 10mM.
Green CMFDA, as a non-terminal, non-fluorescent probe, can be cleaved by non-specific esterases common to living cells, producing a fluorescent compound, fluorescein, visible using a fluorescent microscope[1]. CMFDA is excited with a monochromator at 492 nm and is emissed at 548 nm[2].
In vitro, there was a a positive chondrogenic process of hBM-MSC labeled with 10 μM CellTracker? Green CMFDA, indicating these cells could differentiate into chondrogenic cells similarly to controlled non-labeled cells[3]. In vitro experiment it shown that 4 weeks after grafting stem cells, the green fluorescence of the originally CMFDA-labeled (10 μM) bladder epithelia was still detectable in all of the resulting glandular prostate epithelium[4].
References:
[1] Bernhard, et al. Comparison of two methods to identify live benthic foraminifera: a test between Rose Bengal and CellTracker Green with implications for stable isotope paleoreconstructions. Paleoceanography. 2006;21.
[2] Trontelj K, et al. Cell electrofusion visualized with fluorescence microscopy. J Vis Exp. 2010 Jul 1;(41):1991.
[3] Andrzejewska A, et al. Labeling of human mesenchymal stem cells with different classes of vital stains: robustness and toxicity. Stem Cell Res Ther. 2019 Jun 25;10(1):187.
[4] Li X, et al. Urothelial transdifferentiation to prostate epithelia is mediated by paracrine TGF-beta signaling. Differentiation. 2009 Jan;77(1):95-102.
绿色 CMFDA 作为一种非末端、非荧光探针,可以被活细胞常见的非特异性酯酶切割,产生荧光化合物荧光素,使用荧光显微镜可见[1]。 CMFDA 在 492 nm 处用单色器激发,并在 548 nm 处发射[2]。
在体外,用 10 μM CellTracker™ Green CMFDA 标记的 hBM-MSC 有一个阳性软骨形成过程,表明这些细胞可以像对照的未标记细胞一样分化成软骨形成细胞[3] .体外实验表明,移植干细胞 4 周后,在所有生成的腺体前列腺上皮细胞中仍可检测到最初 CMFDA 标记的 (10 μM) 膀胱上皮细胞的绿色荧光[4]。
| Cell experiment [1]: | |
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Cell lines |
platelets |
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Preparation Method |
Samples of resting platelets and platelets activated by 100 µM ADP were adjusted to the platelet concentration of 3.5~3.8 × 108/L with autologous plasma, and incubated for 30 minutes at 37°C in 10 µM CMFDA. After incubation, the cytosolic esterase-induced fluorescence of platelets was measured by laser confocal microscopy and also photograph was taken. |
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Reaction Conditions |
for 30 minutes at 37°C in 10 µM |
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Applications |
The observation with laser confocal microscopy on CMFDA-stained platelets shows that nearly all the resting platelets release bright green fluorescence, whereas the ADP activated platelets release no or little fluorescence. |
| Animal experiment [2]: | |
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Animal models |
NOD/SCID or NOD/SCID/β2Mnull mice |
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Preparation Method |
To determine the optimal time of BMDC injection in relation to bleo injury, 5 × 106 CMFDA-labeled BMDC were washed, resuspended in Hanks' buffer with heparin (50 U/ml), and administered via tail vein injection to NOD/SCID or NOD/SCID/β2Mnull mice 1, 2, 3, or 4 days post-bleo. |
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Dosage form |
5 × 106 ;1, 2, 3, or 4 days |
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Applications |
There was a 10- to 100-fold increase in the percentage of CMFDA+ cells seen in NOD/SCID/β2Mnull mice compared with NOD/SCID mice, with ∼0.04% positive cells seen when cells were infused at day 4 and mice were killed at day 7 after bleo. |
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References: [1] Wang J, et al. Correlation between the In Vitro Functionality of Stored Platelets and the Cytosolic Esterase-Induced Fluorescence Intensity with CMFDA. PLoS One. 2015 Sep 21;10(9):e0138509. |
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| Cas No. | 136832-63-8 | SDF | |
| 别名 | 5-氯甲基荧光素二乙酸酯,5-Chloromethylfluorescein diacetate | ||
| 化学名 | 3',6'-bis(acetyloxy)-5-(chloromethyl)-spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one | ||
| Canonical SMILES | O=C(O1)C2=C(C=CC(CCl)=C2)C31C4=CC=C(OC(C)=O)C=C4OC5=C3C=CC(OC(C)=O)=C5 | ||
| 分子式 | C25H17ClO7 | 分子量 | 464.9 |
| 溶解度 | 0.1 M Na2CO3: 5 mg/mL,DMF: 25 mg/mL,DMSO: 33 mg/mL,Ethanol: 25 mg/mL,PBS (pH 7.2): 20 µ g/ml | 储存条件 | Store at -20°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.151 mL | 10.755 mL | 21.51 mL |
| 5 mM | 0.4302 mL | 2.151 mL | 4.302 mL |
| 10 mM | 0.2151 mL | 1.0755 mL | 2.151 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
