GSK2200150A
目录号 : GC32317
GSK2200150A is identified by high-throughput screening (HTS) campaign that can be used as an anti-tuberculosis (TB) agent (MIC = 0.38 ?M).
Cas No.:1443138-53-1
Sample solution is provided at 25 µL, 10mM.
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GSK2200150A is identified by high-throughput screening (HTS) campaign that can be used as an anti-tuberculosis (TB) agent (MIC = 0.38 ?M).
GSK2200150A is a novel antimycobacterial agent against Mycobacterium tuberculosis, by inhibiting the growth of virulent M.tuberculosis strain (H37Rv) with MIC of 0.38 μM.[1]
[1] Badiola KA, et al. PLoS One. 2014 Dec 10;9(12):e111782.
Cell experiment: | GSK2200150A is tested for activity at either single concentration (100 µM) or serially diluted in 10 µL of purified H2O in triplicate in 96 well microtiter plates. M. tuberculosis H37Rv is grown in complete Middlebrook 7H9 media containing albumin, dextrose and catalase (ADC), 20% Tween 80 and 50% glycerol. A bacterial suspension (90 µL) at OD600 nm of 0.001 is added to the wells and incubated for 7 days. Resazurin (10 µL; 0.05%(w/v)) is then added, incubated for 24 h at 37°C, and fluorescence measured at 590 nm using a FLUOstar Omega microplate reader. After subtraction of background fluorescence from all wells, the percentage mycobacterial survival is determined by comparing the fluorescence of wells containing compounds compared to control wells not treated with compound[1]. |
References: [1]. Badiola KA, et al. Efficient Synthesis and Anti-Tubercular Activity of a Series of Spirocycles: An Exercise in OpenScience. PLoS One. 2014 Dec 10;9(12):e111782. | |
| Cas No. | 1443138-53-1 | SDF | |
| Canonical SMILES | N1(CC2=CC=C(OCCO3)C3=C2)CCC4(C(C=CS5)=C5CCO4)CC1 | ||
| 分子式 | C20H23NO3S | 分子量 | 357.47 |
| 溶解度 | DMSO : ≥ 83.33 mg/mL (233.11 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.7974 mL | 13.9872 mL | 27.9744 mL |
| 5 mM | 0.5595 mL | 2.7974 mL | 5.5949 mL |
| 10 mM | 0.2797 mL | 1.3987 mL | 2.7974 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet