H-Ala-OH
(Synonyms: L-丙氨酸; L-2-Aminopropionic acid) 目录号 : GA11068
H-Ala-OH 是一种非必需氨基酸，参与糖和酸代谢，提高免疫力，为肌肉组织、大脑和中枢神经系统提供能量。
Cas No.:56-41-7
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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Cell experiment: |
To investigate the selective removal of hiPSCs from differentiated cells by the high L-Alanine medium, two types of hiPSCs, 201B7 hiPSCs (201B7 cells) and an hiPSC line derived by episomal system (ehiPSCs) are used, along with normal human dermal fibroblasts (hFBs), human skeletal muscle cells (hSkMCs) and hiPSC-derived cardiomyocytes (iCMs) as differentiated cells. The cells are incubated in a medium supplemented with L-Alanine at various concentrations (0-1.2 M) or treatment times (1-24 h). The medium is replaced with a normal medium and the relative cell viability is measured after 24 h[1]. |
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References: [1]. Nagashima T, et al. Selective Elimination of Human Induced Pluripotent Stem Cells Using Medium with High Concentration of L-Alanine. Sci Rep. 2018 Aug 20;8(1):12427. |
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L-Alanine is a non-essential amino acid, involved in sugar and acid metabolism, increases immunity, and provides energy for muscle tissue, brain, and central nervous system.
The viability of both hiPSCs, 201B7 cells and ehiPSCs decrease with an increase in L-Alanine concentration, and reach 7.5±1.3% and 3.7±0.7% respectively at 1.2 M of L-Alanine. On the other hand, no decrease in the viability of hFBs and hSkMCs are observed. Although the viability of iCMs slightly decreases along with the increase of the L-Alanine concentration, viability of iCMs at 1.2 M concentration of L-Alanine, 49.4±6.9%, is significantly higher than that of undifferentiated iPSCs, 201B7 cells and ehiPSCs (p< 0.01). The viability of hiPSCs, 201B7 cells and ehiPSCs, drastically decrease even after 2 or 4 h treatment. In contrast, the viability of hFBs fails to decrease at 1, 2, and 4 h and shows a small decrease at 24 h treatment. The viability of 201B7 cells in suspension culture decreases to 11.8±6.0% following treatment with 1.2 M L-Alanine for 2 h, whereas that of hFBs is 72.9±14.2%[1].
References:
[1]. Nagashima T, et al. Selective Elimination of Human Induced Pluripotent Stem Cells Using Medium with High Concentration of L-Alanine. Sci Rep. 2018 Aug 20;8(1):12427.
| Cas No. | 56-41-7 | SDF | |
| 别名 | L-丙氨酸; L-2-Aminopropionic acid | ||
| 化学名 | (2S)-2-aminopropanoic acid | ||
| Canonical SMILES | CC(C(=O)O)N | ||
| 分子式 | C3H7NO2 | 分子量 | 89.1 |
| 溶解度 | PBS (pH 7.2): 10mg/mL | 储存条件 | Desiccate at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 11.2233 mL | 56.1167 mL | 112.2334 mL |
| 5 mM | 2.2447 mL | 11.2233 mL | 22.4467 mL |
| 10 mM | 1.1223 mL | 5.6117 mL | 11.2233 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。