H-D-Met-OH
(Synonyms: D-蛋氨酸,MRX-1024; D-Methionine) 目录号 : GA10004
H-D-Met-OH (MRX-1024; D-H-D-Met-OH) 是一种有效的化学保护剂，还可以通过 GABAA 受体激活抑制神经元活性。
Cas No.:348-67-4
Sample solution is provided at 25 µL, 10mM.
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Methionine (MRX-1024; D-Methionine) is an effective chemoprotective agent which can also inhibit the neuronal activity through GABAA receptor activation.
The incubation of human plasma with Methionine (D-methionine) and CP leads to the formation of a Pt-D-methionine complex independent of the order of addition. In plasma, an early CP hydrolysis product reacts with Methionine to form a 1:1 complex that is followed by the formation of a 2:1 compound at a later time point. The formation of these Pt-D-methionine species plays an important role in the processes by which Methionine protects mammalian organisms against CP-induced toxicities[2].
When Methionine (D-methionine) is administered along with cisplatin the cell density is 0.8±0.070 (SEM), significantly larger than in rats treated with only cisplatin (P0.05). When Methionine is administered alone the average cell density is 0.95±0.099 (SEM) and not significantly different from controls (P>0.05)[3].
References:
[1]. Wu C, et al. Antioxidants L-carnitine and D-methionine modulate neuronal activity through GABAergic inhibition. J Neural Transm (Vienna). 2014 Jul;121(7):683-93.
[2]. Sooriyaarachchi M, et al. Chemoprotection by D-methionine against cisplatin-induced side-effects: insight from in vitrostudies using human plasma. Metallomics. 2014 Mar;6(3):532-41.
[3]. Hinduja S, et al. D-methionine protects against cisplatin-induced neurotoxicity in the hippocampus of the adult rat. Neurotox Res. 2015 Apr;27(3):199-204.
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Cell experiment: |
Plasma (2.0 mL) is spiked with 81 mL of the CP stock solution, incubated at 37°C for 30 min and then 20 mL of theMethionine (D-methionine) stock solution (40.7 mg/mL) is added. The obtained mixture is analyzed after 10 min and 50 min of incubation at 37°C[2]. |
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Animal experiment: |
Three sham controls receive 0.9% saline instead of cisplatin. Three rats receive only cisplatin. Another three rats receive Methionine (D-methionine) 30 minutes prior to treatment with cisplatin. Injection of Methionine prior to cisplatin treatment ensures that it is already taken up before cisplatin is applied. Finally, three rats are treated only with Methionine. All 12 rats are sacrificed at day 7 post-treatment. For prescreening, two further rats are treated with cisplatin alone and sacrificed 2 or 21 days later. All rats are hydrated with 0.9% saline[3]. |
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References: [1]. Wu C, et al. Antioxidants L-carnitine and D-methionine modulate neuronal activity through GABAergic inhibition. J Neural Transm (Vienna). 2014 Jul;121(7):683-93. |
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| Cas No. | 348-67-4 | SDF | |
| 别名 | D-蛋氨酸,MRX-1024; D-Methionine | ||
| 化学名 | (2R)-2-amino-4-methylsulfanylbutanoic acid | ||
| Canonical SMILES | CSCCC(C(=O)O)N | ||
| 分子式 | C5H11NO2S | 分子量 | 149.2 |
| 溶解度 | Water : 29mg/mL | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 6.7024 mL | 33.5121 mL | 67.0241 mL |
| 5 mM | 1.3405 mL | 6.7024 mL | 13.4048 mL |
| 10 mM | 0.6702 mL | 3.3512 mL | 6.7024 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet