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HARS Human, Sf9

目录号 : GP21777

Histidyl-tRNA Synthetase Human Recombinant, Sf9

HARS Human, Sf9 Chemical Structure

规格 价格 库存 购买数量
5μg
¥840.00
5-10工作日
25μg
¥2,030.00
5-10工作日
1mg
¥54,600.00
5-10工作日

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Sample solution is provided at 25 µL, 10mM.

Description

Histidyl-tRNA Synthetase Human Recombinant produced in baculovirus is a single, glycosylated, polypeptide chain having a molecular mass of 58.3 kDa.The Histidyl-tRNA Synthetase is fused to 6x His Tag and purified by proprietary chromatographic techniques.

Product Data

Purity Greater than 90.0% as determined by SDS-PAGE. Source Sf9 Insect Cells.
Phycical Appearance Sterile Filtered clear solution. Shipping Condition Shipped with Ice Packs.
Synonyms Histidyl-tRNA synthetase; EC 6.1.1.21; Histidine-tRNA ligase; HisRS; HRS; FLJ20491; JO-1.
Stability Histidyl-tRNA Synthetase although stable at 4°C for 3 weeks, should be stored below -18°C .Please prevent freeze-thaw cycles.
Formulation The protein solution contains 20mM HEPES, 250mM sodium chloride 0.1% and 20% Glycerol, (pH 7.5).

Introduction

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.

Applications

Western Blot: Strongly reactive with human anti Histidyl-tRNA Synthetase antisera.

Stability

Histidyl-tRNA Synthetase although stable at 4°C for 3 weeks, should be stored below -18°C .Please prevent freeze-thaw cycles.

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