Hematoporphyrin (Hematoporphyrin IX)
(Synonyms: 血卟啉; Hematoporphyrin IX) 目录号 : GC30041
血卟啉（Hematoporphyrin IX）（Hematoporphyrin (Hematoporphyrin IX) IX）是一种光敏剂，是血红素结合蛋白亲和层析的底物。血卟啉 (Hematoporphyrin IX) 可在暴露于红光时诱导 U87 胶质瘤细胞凋亡并减少体内肿瘤生长。
Cas No.:14459-29-1
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Hematoporphyrin (Hematoporphyrin IX) is a substrate for affinity chromatography of heme-binding proteins.
Hematoporphyrin is used for affinity chromatography of heme-binding proteins. Hematoporphyrin is used in the characterization and synthesis of hematoporphyrin- and hematin-agarose [1].
[1]. Olsen, K.W., Affinity chromatography of heme-binding proteins: synthesis and characterization of hematin- and hematoporphyrin-agarose. Methods Enzymol, 1986. 123: p. 324-31.
| Cas No. | 14459-29-1 | SDF | |
| 别名 | 血卟啉; Hematoporphyrin IX | ||
| Canonical SMILES | O=C(O)CCC1=C2/C=C3C(CCC(O)=O)=C(C)C(/C=C(N/4)/C(C)=C(C(O)C)C4=C\C5=N/C(C(C(O)C)=C5C)=C\C(N2)=C1C)=N/3 | ||
| 分子式 | C34H38N4O6 | 分子量 | 598.69 |
| 溶解度 | DMSO : 150 mg/mL (250.55 mM);Water : < 0.1 mg/mL (insoluble) | 储存条件 | -20°C, protect from light,unstable in solution, ready to use. |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.6703 mL | 8.3516 mL | 16.7031 mL |
| 5 mM | 0.3341 mL | 1.6703 mL | 3.3406 mL |
| 10 mM | 0.167 mL | 0.8352 mL | 1.6703 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Photosensitizing effect of Hematoporphyrin IX on immature stages of Ceratitis capitata (Diptera: Tephritidae)
Photochem Photobiol 2010 May-Jun;86(3):639-44.20202159 10.1111/j.1751-1097.2009.00704.x
Immature stages of Ceratitis capitata were tested as a model for Hematoporphyrin IX (HP IX) phototoxicity. The lethal concentration 50 (LC(50)) of HP IX in the food was determined during postembryonic development until adult emergence as 0.173 mm (95% CI: 0.138-0.209). The corresponding HP IX LC(50) during the dispersal period alone was 0.536 mm (95% CI: 0.450-0.633). HP IX toxicity was compared against Phloxine B (PhB) (0.5 mm). HP IX elicited a mortality of 90.87%, which was mainly concentrated during prepupal and early pupal stages. PhB mortality was much lower (56.88%) and occurred mainly during the adult pharate stage. A direct correlation between light-dependent HP IX mortality, evidence of reactive oxygen species (ROS) and lipid peroxidation (conjugated dienes and thiobarbituric acid reactive substances) was established in C. capitata larvae. ROS were found to be very significant in both the brain and in the gut.
Cellular Pharmacology of Palladinum(III) Hematoporphyrin IX Complexes: Solution Stability, Antineoplastic and Apoptogenic Activity, DNA Binding, and Processing of DNA-Adducts
Int J Mol Sci 2018 Aug 19;19(8):2451.30126243 PMC6121444
Two paramagnetic PdIII complexes of Hematoporphyrin IX ((7,12-bis(1-hydroxyethyl)-3,8,13,17-tetramethyl-21H-23H-porphyn-2,18-dipropionic acid), Hp), namely a dinuclear one [PdIII₿Hp-3H)Cl₿H₂O)₅]·2PdCl₿ Pd1 and a mononuclear metalloporphyrin type [PdIII(Hp-2H)Cl(H₂O)]·H₂O, Pd2 have been synthesized reproducibly and isolated as neutral compounds at different reaction conditions. Their structure and solution stability have been assayed by UV/Vis and EPR spectroscopy. The compounds researched have shown in vitro cell growth inhibitory effects at micromolar concentration against a panel of human tumor cell lines. A DNA fragmentation test in the HL-60 cell line has indicated that Pd1 causes comparable proapoptotic effects with regard to cisplatin but at substantially higher concentrations. Pd1 and cisplatin form intra-strand guanine bis-adducts as the palladium complex is less capable of forming DNA adducts. This demonstrates its cisplatin-dissimilar pharmacological profile. The test for efficient removal of DNA-adducts by the NER synthesis after modification of pBS plasmids with either cisplatin or Pd1 has manifested that the lesions induced by cisplatin are far better recognized and repaired compared those of Pd1. The study on the recognition and binding of the HMGB-1 protein to cisplatin or Pd1 modified DNA probes have shown that HMG proteins are less involved in the palladium agent cytotoxicity.
Excited state absorption study in Hematoporphyrin IX
J Fluoresc 2010 Jan;20(1):197-202.19756981 10.1007/s10895-009-0538-z
We present the study of the excited state absorption of Hematoporphyrin IX dissolved in dimethyl sulfoxide. All measurements were carried out using open aperture Z-scan and white-light continuum pump-probe with picosecond pulses to avoid triplet excited state absorption. Without the latter contribution, the results obtained with both techniques show a transition to a high singlet excited state. The vibronic progression of the Q-band is observed due to photobleaching of the ground state. In addition, we show that the excited state presents reverse saturable absorption for most of the spectral range studied. A long relaxation component of the first singlet excited state was evidenced with the pump-probe experiment. This result is in agreement with fluorescence lifetime and fluorescence quantum yield measurements. In order to elucidate the origin of the nonlinear effects, we used a three-level energy diagram to describe the principal singlet-singlet transitions.
Comparision between sonodynamic effects with protoporphyrin IX and Hematoporphyrin on the cytoskeleton of Ehrlich ascites carcinoma cells
Cancer Biother Radiopharm 2010 Feb;25(1):55-64.20187797 10.1089/cbr.2008.0604
Purpose: Our previous work demonstrated that the enhancement of cytotoxicity by protoporphyrin IX (Pp) was significantly higher than Hematoporphyrin (Hp), when they were compared at the same ultrasound-exposure conditions, since the cytoskeleton plays a crucial role in numerous cell functions and the effect of sonodynamic therapy (SDT) on cytoskeleton has not been reported yet. So, it is very important to investigate whether SDT has some influence on the cytoskeleton, and it would also be interesting to compare Pp-/ and Hp-mediated SDT on the cytoskeleton. Methods: Ehrlich ascites carcinoma (EAC) cells were exposed to ultrasound for up to 15 seconds (1.34 MHz, 1 w/cm(2)) in the presence of 20 microM of Pp or 20 microM of Hp. Cell viability was determined by the trypan blue exclusion test. Morphologic changes were observed under a scanning electron microscope. Cytoskeleton damages were shown by immunofluorescence staining of microtubules, actin microfilaments, and intermediate filaments of vimentin. Results: Our experiment showed that the extent of cytoskeleton damage in EAC cells increased as time was prolonged, and also which was more significant in the Pp-SDT group than that in the Hp-SDT group at the same time after exposure. Conclusions: Results implied that the ultrastructural changes might account for cell destruction and the cytoskeletal components were involved in the cell-damaging process induced SDT treatment.
Comparison between sonodynamic effect with protoporphyrin IX and Hematoporphyrin on sarcoma 180
Cancer Chemother Pharmacol 2007 Oct;60(5):671-80.17219011 10.1007/s00280-006-0413-4
Purpose: The comparison between sonodynamic antitumor effect with protoporphyrin IX (PPIX) and Hematoporphyrin (Hp) at a concentration of 5 mg/kg on Sarcoma 180 (S180) cells was studied in vivo, and the potential cell damage mechanism was also investigated. Methods: The sonodynamically induced anti-tumor effect of PPIX was studied in mice bearing S180 solid tumors. In order to determine the optimum timing of ultrasound exposure after administration of PPIX, the PPIX concentrations in plasma, skin, muscle and tumor were determined by the fluorescence intensity of tissue extractions with a fluorescence spectrophotometer based on the standard curve. Anti-tumor effects were estimated by measuring the tumor size and the tumor weight. Additionally, the morphological changes of S180 cells were evaluated by transmission electron microscope (TEM) observation immediately after sonodynamic therapy (SDT) treatment. Results: A time of 24 h after the intravenous administration of PPIX was chosen as the best time for ultrasound exposure. The antitumor effect induced by PPIX mediated sonodynamic therapy (PPIX-SDT) was in a dose dependent manner when ultrasound intensity was at or above the inertial cavitation threshold (5 W/cm(2)). A significant tumor growth delay was observed both in PPIX mediated sonodynamic therapy and in Hp mediated sonodynamic therapy treatments (Hp-SDT), and the tumor weight inhibition ratios after the synergistic treatments were 42.82 +/- 0.03 and 35.22 +/- 0.03%, respectively, this difference was significant at P < 0.05. While ultrasound alone (5 W/cm(2)) showed a slight tumor growth inhibitory effect compared with the control group, and PPIX or Hp alone showed almost no significant effect. Furthermore, TEM observation indicated cell damage was more serious in PPIX-SDT treatment group than in Hp-SDT treatment group. After sonication, the cell ultra-structure such as cell membrane destruction, mitochondria swelling, chromatin condensation might be important factors that inhibited the tumor growth and even induced cell death. Conclusions: The comparative results suggested that PPIX as a sonosensitizer might have more potential cytotoxicity than Hp when irradiated with ultrasound, and the ultra-structural changes may account for cell destruction induced by sonodynamic therapy in our experiment mode.