Heparan Sulfate
(Synonyms: 硫酸乙酰肝素) 目录号 : GC30767
硫酸乙酰肝素 (HS) 是一种复杂的聚阴离子多糖，广泛表达于细胞表面和细胞外基质。
Cas No.:9050-30-0
Sample solution is provided at 25 µL, 10mM.
Heparan sulfate (HS) is a complex, polyanionic polysaccharide ubiquitously expressed on cell surfaces and in the extracellular matrix[1]. Heparan sulfate interacts with numerous proteins, including growth factors, morphogens, and adhesion molecules, and thereby regulates important developmental processes in invertebrates and vertebrates[6]. Heparan sulfate proteoglycans can act as receptors for proteases and protease inhibitors regulating their spatial distribution and activity. Membrane Heparan sulfate proteoglycans act as coreceptors for various tyrosine kinase-type growth factor receptors, lowering their activation threshold or changing the duration of signaling reactions[5].
Heparan sulfate treatment significantly reduced SARS-CoV-2 replication in cell lysates and supernatant samples of Calu3 and Caco2. HS serves as an essential host determinant during SARS-CoV-2 attachment and replication[2].Heparan sulfate influences the binding affinity of intestinal epithelium cells to Wnt, thereby promoting activation of canonical Wnt signaling and facilitating regeneration of small intestinal crypts after epithelial injury[7].
In Sprague-Dawley male rats, FGF-2/FGFR system is involved in the regulation of astrocytic reactivity and/or proliferation in the brain and its action is potentiated by Heparan sulfate [3].Heparan sulfate proteoglycans represent a major component of the extracellular matrix and are critical for brain development, Heparan Sulfate can maintain neuronal excitability, promote synaptic plasticity and learning[5].
References:
[1]: Chhabra M, Doherty GG, et,al. From Cancer to COVID-19: A Perspective on Targeting Heparan Sulfate-Protein Interactions. Chem Rec. 2021 Nov;21(11):3087-3101. doi: 10.1002/tcr.202100125. Epub 2021 Jun 19. PMID: 34145723; PMCID: PMC8441866.
[2]: Chu H, Hu B, et,al. Host and viral determinants for efficient SARS-CoV-2 infection of the human lung. Nat Commun. 2021 Jan 8;12(1):134. doi: 10.1038/s41467-020-20457-w. PMID: 33420022; PMCID: PMC7794309.
[3]: Gómez-Pinilla F, Vu L, et,al. Regulation of astrocyte proliferation by FGF-2 and heparan sulfate in vivo. J Neurosci. 1995 Mar;15(3 Pt 1):2021-9. doi: 10.1523/JNEUROSCI.15-03-02021.1995. PMID: 7891149; PMCID: PMC6578134.
[4]: Minge D, Senkov O, et,al. Heparan Sulfates Support Pyramidal Cell Excitability, Synaptic Plasticity, and Context Discrimination. Cereb Cortex. 2017 Feb 1;27(2):903-918. doi: 10.1093/cercor/bhx003. PMID: 28119345; PMCID: PMC5390399.
[5]: Sarrazin S, Lamanna WC, et,al. Heparan sulfate proteoglycans. Cold Spring Harb Perspect Biol. 2011 Jul 1;3(7):a004952. doi: 10.1101/cshperspect.a004952. PMID: 21690215; PMCID: PMC3119907.
[6]: Kraushaar DC, Dalton S, et,al. Heparan sulfate: a key regulator of embryonic stem cell fate. Biol Chem. 2013 Jun;394(6):741-51. doi: 10.1515/hsz-2012-0353. PMID: 23370908; PMCID: PMC3933957.
[7]: Yamamoto S, Nakase H, et,al. Heparan sulfate on intestinal epithelial cells plays a critical role in intestinal crypt homeostasis via Wnt/β-catenin signaling. Am J Physiol Gastrointest Liver Physiol. 2013 Aug 1;305(3):G241-9. doi: 10.1152/ajpgi.00480.2012. Epub 2013 Jun 6. PMID: 23744737; PMCID: PMC3742857.
硫酸乙酰肝素 (HS) 是一种复杂的聚阴离子多糖,广泛表达于细胞表面和细胞外基质[1]。硫酸乙酰肝素与许多蛋白质相互作用,包括生长因子、形态发生素和粘附分子,从而调节无脊椎动物和脊椎动物的重要发育过程[6]。硫酸乙酰肝素蛋白聚糖可以作为蛋白酶和蛋白酶抑制剂的受体,调节它们的空间分布和活性。膜硫酸乙酰肝素蛋白多糖作为多种酪氨酸激酶型生长因子受体的辅助受体,降低其激活阈值或改变信号反应的持续时间[5]。
硫酸乙酰肝素处理显着降低了 Calu3 和 Caco2 细胞裂解物和上清液样品中的 SARS-CoV-2 复制。 HS 在 SARS-CoV-2 附着和复制过程中作为重要的宿主决定因素[2]。硫酸乙酰肝素影响肠上皮细胞与 Wnt 的结合亲和力,从而促进经典 Wnt 信号通路的激活并促进再生上皮损伤后小肠隐窝的形成[7].
在 Sprague-Dawley 雄性大鼠中,FGF-2/FGFR 系统参与脑内星形胶质细胞反应性和/或增殖的调节,硫酸乙酰肝素可增强其作用[3]。硫酸乙酰肝素蛋白多糖是细胞外基质的主要成分,对大脑发育至关重要,硫酸乙酰肝素可维持神经元兴奋性,促进突触可塑性和学习[5]。
| Cell experiment [1]: | |
|
Cell lines |
Calu3 (lung epithelium) and Caco2 (intestinal epithelium) cells |
|
Preparation Method |
Calu3 (lung epithelium) and Caco2 (intestinal epithelium) cells were infected with early passage SARS-CoV-2 pretreated with Heparan sulfate, and samples were collected 24 hours after infection. |
|
Reaction Conditions |
0-500ug/ml Heparan sulfate,24h |
|
Applications |
Heparan sulfate treatment significantly reduced SARS-CoV-2 replication in cell lysates and supernatant samples of Calu3 and Caco2. |
| Animal experiment [2]: | |
|
Animal models |
Sprague-Dawley male rats 3-4 months old |
|
Preparation Method |
Groups of rats (n = 5) received a piece of Gelfoam embedded with human recombinant FGF-2 dissolved in PBS, Heparan sulfate (HS; 10 μg/ml) dissolved in PBS, or both, in the right hemispheres. |
|
Dosage form |
A piece of Gelfoam embedded with 10 ug/ml Heparan sulfate for two days |
|
Applications |
FGF-2/FGFR system is involved in the regulation of astrocytic reactivity and/or proliferation in the brain and its action is potentiated by Heparan sulfate. |
|
References: [1]. Chu H, Hu B, et,al. Host and viral determinants for efficient SARS-CoV-2 infection of the human lung. Nat Commun. 2021 Jan 8;12(1):134. doi: 10.1038/s41467-020-20457-w. PMID: 33420022; PMCID: PMC7794309. [2]. Gómez-Pinilla F, Vu L, et,al. Regulation of astrocyte proliferation by FGF-2 and heparan sulfate in vivo. J Neurosci. 1995 Mar;15(3 Pt 1):2021-9. doi: 10.1523/JNEUROSCI.15-03-02021.1995. PMID: 7891149; PMCID: PMC6578134. |
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| Cas No. | 9050-30-0 | SDF | |
| 别名 | 硫酸乙酰肝素 | ||
| Canonical SMILES | O=S(OCC1O[C@@H](OC)[C@@H](NS(=O)([O-])=O)[C@@H](OS(=O)([O-])=O)[C@H]1O[C@@H]2OC(C([O-])=O)[C@H](O[C@@H]3OC(COS(=O)([O-])=O)[C@H](O[C@@H]4OC(C([O-])=O)[C@H](OC)[C@H](O)[C@@H]4OS(=O)([O-])=O)[C@H](O)[C@@H]3NS(=O)([O-])=O)[C@H](O)[C@@H]2O)([O-])=O.[n] | ||
| 分子式 | C12H19NO20S3 (monomer) | 分子量 | 593.47(monomer) |
| 溶解度 | Water : 47.1 mg/mL | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 1.685 mL | 8.425 mL | 16.8501 mL |
| 5 mM | 0.337 mL | 1.685 mL | 3.37 mL |
| 10 mM | 0.1685 mL | 0.8425 mL | 1.685 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
