Hexa-N-acetylchitohexaose
(Synonyms: N,N',N,N''',N'''',N'''''-六乙酰壳六糖,N-Acetylchitohexaose，NACOS-6) 目录号 : GC43822
A hexamer of N-acetylglucosamine
Cas No.:38854-46-5
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
Hexa-N-acetylchitohexaose is a hexamer of N-acetylglucosamine, a subunit of the natural polymer chitin. It functions as an elicitor in plants, inducing the expression of chitinases. [1][2] Like chitin and some of its derivatives, hexa-N-acetylchitohexaose is a substrate of lysozyme.[3] It also binds LysM domains on certain proteins, including an endopeptidase of T. thermophilus [4]. Hexa-N-acetylchitohexaose heightens the immune response against Pseudomonas and Listeria in mice, stimulates cytokine secretion in mesenchymal stem cells, and inhibits nitric oxide production by activated macrophages.[5][6][7][8]
Reference:
[1]. Inui, H., Yamaguchi, Y., and Hirano, S. Elicitor actions of N-acetylchitooligosaccharides and laminarioligosaccharides for chitinase and L-phenylalanine ammonia-lyase induction in rice suspension culture. Bioscience, Biotechnology, and Biochemistry 61(6), 975-978 (1997).
[2]. Gao, Y., Zan, X.L., Wu, X.F., et al. Identification of fungus-responsive cis-acting element in the promoter of Brassica juncea chitinase gene, BjCHI1. Plant Science 215-216, 190-198 (2014).
[3]. Ouyang, Z., Takáts, Z., Blake, T.A., et al. Preparing protein microarrays by soft-landing of mass-selected ions. Science 301, 1351-1354 (2003).
[4]. Wong, J.E.M.M., Midtgaard, S.R., Gysel, K., et al. An intermolecular binding mechanism involving multiple LysM domains mediates carbohydrate recognition by an endopeptidase. Acta.Cryst. D71, 592-605 (2014).
[5]. Tokoro, A., Kobayashi, M., Tatewaki, N., et al. Protective effect of N-acetyl chitohexaose on Listeria monocytogenes infection in mice. Microbiology and Immunology 33(4), 357-367 (1989).
[6]. Okawa, Y., Kobayashi, M., Suzuki, S., et al. Comparative study of protetive effects of chitin, chitosan, and N-acetyl chitohexaose against Pseudomonas aeruginosa and Listeria monocytogenes infections in mice. Biological and Pharmaceutical Bullentin 26(6), 902-904 (2003).
[7]. Lieder, R., Thormodsson, F., Ng, C.H., et al. Chitosan and chitin hexamers affect expansion and differentiation of mesenchymal stem cells differently. Int.J.Biol.Macromol. 51(4), 675-680 (2012).
[8]. Hwang, S.M., Chen, C.Y., Chen, S.S., et al. Chitinous materials inhibit nitric oxide production by activated RAW 264.7 macrophages. Biochemical and Biophysical Research Communications 271(1), 229-233 (2000).
| Cas No. | 38854-46-5 | SDF | |
| 别名 | N,N',N,N''',N'''',N'''''-六乙酰壳六糖,N-Acetylchitohexaose，NACOS-6 | ||
| 化学名 | O-2-(acetylamino)-2-deoxy-β-D-glucopyranosyl-(1→4-O-2-(acetylamino)-2-deoxy-β-D-glucopyranosyl-(1→4)-O-2-(acetylamino)-2-deoxy-β-D-glucopyranosy-(1→4)-O-2-(acetylamino)-2-deoxy-β-D-glucopyranosyl-(1→4)-O-2-(acetylamino)-2-deoxy-β-D-glucopyranosyl-(1→4)-2-(acetylamino)-2-deoxy-D-glucose | ||
| Canonical SMILES | OC[C@H]1O[C@](O[C@@H]2[C@@H](CO)O[C@](O[C@H]3[C@H](O)[C@@H](NC(C)=O)[C@H](O[C@]4([H])[C@@H](CO)O[C@@H](O[C@@]5([H])[C@H](O)[C@@H](NC(C)=O)[C@H](O[C@@]([C@H](O)CO)([H])[C@H](O)[C@@H](NC(C)=O)C=O)O[C@@H]5CO)[C@H](NC(C)=O)[C@H]4O)O[C@@H]3CO)([H])[C@H](N | ||
| 分子式 | C48H80N6O31 | 分子量 | 1237.2 |
| 溶解度 | 10mg/mL in PBS, pH 7.2 | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 0.8083 mL | 4.0414 mL | 8.0828 mL |
| 5 mM | 0.1617 mL | 0.8083 mL | 1.6166 mL |
| 10 mM | 0.0808 mL | 0.4041 mL | 0.8083 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet