Homoplantaginin
(Synonyms: 高车前苷) 目录号 : GC31692
A flavonoid glycoside with antioxidant and anti-inflammatory activities
Cas No.:17680-84-1
Sample solution is provided at 25 µL, 10mM.
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Homoplantaginin is a flavonoid glycoside that has been found in S. plebeia and has antioxidant and anti-inflammatory activities.1,2,3 It scavenges 2,2-diphenyl-1-picrylhydrazyl radicals with an IC50 value of 0.35 ?g/ml in a cell-free assay.2 Homoplantaginin reverses hydrogen peroxide-induced decreases in glutathione (GSH) levels and glutathione peroxidase (GPX) and superoxide dismutase (SOD) activity in HL-7702 cells when used at concentrations ranging from 1 to 100 ?g/ml. It reduces protein levels of toll-like receptor 4 (TLR4), MyD88, caspase-1, and IL-1β induced by palmitic acid in human umbilical vein endothelial cells (HUVECs) when used at concentrations of 1 and 10 ?M.3 Homoplantaginin (50 and 100 mg/kg) decreases serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), TNF-α, and IL-1 levels, as well as reduces liver inflammatory cell infiltration and hepatocyte necrosis in a mouse model of immunological liver injury induced by Bacillus Calmette-Guérin (BCG) vaccine and LPS.2
1.Weng, X.C., and Weng, W.Antioxidant activity of compounds isolated from Salvia plebiaFood Chem.71(4)489-493(2000) 2.Qu, X.-J., Xia, X., Wang, Y.-S., et al.Protective effects of Salvia plebeia compound homoplantaginin on hepatocyte injuryFood Chem. Toxicol.47(7)1710-1715(2009) 3.He, B., Zhang, B., Wu, F., et al.Homoplantaginin inhibits palmitic acid-induced endothelial cells inflammation by suppressing TLR4 and NLRP3 inflammasomeJ. Cardiovasc. Pharmacol.67(1)93-101(2016)
Cell experiment: | The viability of cultured cells is determined using the MTT assay. Human umbilical vein endothelial cells are exposed to various concentrations of homoplantaginin (0.1, 1, 3, 10, 30, 100 μM) for 48 h. Subsequently, 20 μL of MTT (5 mg/mL) is added to each well for an additional 4 h at 37°C. The supernatant is removed, and DMSO is added to dissolvethe formazan crystals. The optical absorbance is measured at 540 nm[3]. |
Animal experiment: | Rats: Homoplantaginin is dissolved in a solution consistingof DMSO, PEG 400, ethanol and normal saline(2:2:3:3, v/v/v/v) at a concentration of 10 mg/mL. The rats are randomly divided into three groups to receive oral administration(150 mg/kg), tail vein injection (15 mg/kg) and peritoneal injectionv (15 mg/kg). Blood samples (approximately 0.5 mL) are collected from the retro-orbital plexus into heparinized microfugetubes at 5, 10, 20, 30, 45, 60, 90, 120, and 180 min after administration. The plasma samples, separated by centrifuging the blood samples at 10,000 rpm[4]. Mice: Homoplantaginin is dissolved in 5% amylum. Homoplantaginin is administered orally by gastric intubation during the experimental period at doses of 25, 50, 100 mg/kg/d, respectively. Eight hours after injection of LPS, the mice are anesthetized with ether and blood samples are collected by exsanguination from the inferior vein. The liver is removed and fixed in formalin for histological analysis[1]. |
References: [1]. Qu XJ, et al. Protective effects of Salvia plebeia compound homoplantaginin on hepatocyte injury. Food Chem Toxicol. 2009 Jul;47(7):1710-5. | |
| Cas No. | 17680-84-1 | SDF | |
| 别名 | 高车前苷 | ||
| Canonical SMILES | OC1=C(C2=O)C(OC(C3=CC=C(O)C=C3)=C2)=CC(O[C@@H]([C@@H]([C@@H](O)[C@@H]4O)O)O[C@@H]4CO)=C1OC | ||
| 分子式 | C22H22O11 | 分子量 | 462.4 |
| 溶解度 | DMSO : ≥ 100 mg/mL (216.26 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.1626 mL | 10.8131 mL | 21.6263 mL |
| 5 mM | 0.4325 mL | 2.1626 mL | 4.3253 mL |
| 10 mM | 0.2163 mL | 1.0813 mL | 2.1626 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet