Humanin
(Synonyms: 护脑素(人体)) 目录号 : GC18324
Humanin (HN) 是一种天然的短线粒体多肽，具有抗细胞凋亡和神经保护作用。
Cas No.:330936-69-1
Sample solution is provided at 25 µL, 10mM.
Humanin (HN) is a natural short mitochondrial polypeptide with antiapoptotic and neuroprotective effects[2]. Humanin protect cells against various diseases, including oxidative stress and cytotoxicity induced by various stimuli[3] with broad-spectrum activity on pancreas, brain, testis, and heart[4]. Humanin and its analogs could be used as good therapeutic targets against neuroprotective and neurodegenerative diseases[1].
Humanin increased cell viability. HN decreased the percentage of hypodiploid KGN cells as well as the percentage of apoptotic KGN cells. Humanin could be considered a pro-survival factor in this tumor cell line[5]. Humanin may be involved in the response of HLECs to oxidative stress, and that HN expression was significantly upregulated under oxidative stress conditions. Furthermore, exogenous HN reduced intracellular ROS content and mitochondrial damage, and enhanced mitochondrial biosynthesis; however, this protection was lost in an endogenous Humanin knockdown cell model[6]. The interaction of Humanin and its receptors (CNTFR, WSX-1, and gp130) could activate the STAT3 signaling pathway via an increase in STAT3 expression and consequently lead to the increase of beta cell survival in the pancreas[9].
Humanin administration has neuroprotective effects in vitro in human cell culture models and is sufficient to improve cognition in vivo in aged mice[8]. To understand the effects of Humanin on brain function, The effect of S14G-HN on diazepam (DZP)-induced memory impairment and anxiety in mice using the object recognition test and zero-maze test. Intraperitoneal injection of S14G-HN reversed the DZP-induced memory deficit, whereas no significant change was observed in behavioral markers of anxiety. S14G- Humanin had no effect on locomotor activity in either test, indicating that S14G-HN did not affect physical functioning or motivation[7].
References:
[1]. Hazafa A, Batool A, et,al. Humanin: A mitochondrial-derived peptide in the treatment of apoptosis-related diseases. Life Sci. 2021 Jan 1;264:118679. doi: 10.1016/j.lfs.2020.118679. Epub 2020 Oct 29. PMID: 33130077.
[2]. Salemi M, Ridolfo F, et,al.Humanin gene expression in fibroblast of Down syndrome subjects. Int J Med Sci. 2020 Jan 18;17(3):320-324. doi: 10.7150/ijms.39145. PMID: 32132866; PMCID: PMC7053358.
[3]. Luciano F, Zhai D, et,al.Cytoprotective peptide humanin binds and inhibits proapoptotic Bcl-2/Bax family protein BimEL. J Biol Chem. 2005 Apr 22;280(16):15825-35. doi: 10.1074/jbc.M413062200. Epub 2005 Jan 20. PMID: 15661735.
[4]. Paharkova V, Alvarez G, et,al. Rat Humanin is encoded and translated in mitochondria and is localized to the mitochondrial compartment where it regulates ROS production. Mol Cell Endocrinol. 2015 Sep 15;413:96-100. doi: 10.1016/j.mce.2015.06.015. Epub 2015 Jun 23. PMID: 26116236.
[5]. Marvaldi C, Martin D, et,al. Mitochondrial humanin peptide acts as a cytoprotective factor in granulosa cell survival. Reproduction. 2021 Apr 20;161(5):581-591. doi: 10.1530/REP-20-0197. PMID: 33764899.
[6]. Yang H, Cui Y, et,al.Cytoprotective role of humanin in lens epithelial cell oxidative stress?induced injury. Mol Med Rep. 2020 Aug;22(2):1467-1479. doi: 10.3892/mmr.2020.11202. Epub 2020 Jun 2. PMID: 32627019; PMCID: PMC7339735.
[7]. Murakami M, Nagahama M, et,al. Humanin ameliorates diazepam-induced memory deficit in mice. Neuropeptides. 2017 Apr;62:65-70. doi: 10.1016/j.npep.2016.10.008. Epub 2016 Oct 29. PMID: 27814910.
[8]. Yen K, Wan J, et,al.Humanin Prevents Age-Related Cognitive Decline in Mice and is Associated with Improved Cognitive Age in Humans. Sci Rep. 2018 Sep 21;8(1):14212. doi: 10.1038/s41598-018-32616-7. PMID: 30242290; PMCID: PMC6154958.
[9] .Charununtakorn ST, Shinlapawittayatorn K, et,al.Potential Roles of Humanin on Apoptosis in the Heart. Cardiovasc Ther. 2016 Apr;34(2):107-14. doi: 10.1111/1755-5922.12168. PMID: 26667157.
Humanin (HN) 是一种天然的短线粒体多肽，具有抗细胞凋亡和神经保护作用[2]。 Humanin 保护细胞免受各种疾病的侵害，包括氧化应激和各种刺激引起的细胞毒性[3]，对胰腺、大脑、睾丸和心脏具有广谱活性[4] . Humanin 及其类似物可作为神经保护和神经退行性疾病的良好治疗靶点[1]。
Humanin 可提高细胞活力。 HN 降低了亚二倍体 KGN 细胞的百分比以及凋亡 KGN 细胞的百分比。 Humanin 可被视为该肿瘤细胞系的促存活因子[5]。 Humanin 可能参与 HLEC 对氧化应激的反应，并且 HN 表达在氧化应激条件下显着上调。此外，外源性 HN 可降低细胞内 ROS 含量和线粒体损伤，并增强线粒体生物合成；然而，这种保护在内源性 Humanin 敲低细胞模型中丢失了[6]。 Humanin 与其受体（CNTFR、WSX-1 和 gp130）的相互作用可通过增加 STAT3 表达激活 STAT3 信号通路，从而导致胰腺中 β 细胞存活率增加[9].
Humanin 在体外人类细胞培养模型中具有神经保护作用，足以改善老年小鼠体内的认知能力[8]。为了解 Humanin 对脑功能的影响，S14G-HN 对地西泮 (DZP) 诱导的小鼠记忆障碍和焦虑的影响使用物体识别测试和零迷宫测试。腹膜内注射 S14G-HN 可逆转 DZP 诱导的记忆缺陷，而在焦虑的行为标志物中未观察到显着变化。在这两项测试中，S14G-护脑素对运动活动均无影响，表明 S14G-HN 不影响身体机能或动机[7]。
| Cell experiment [1]: | |
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Cell lines |
KGN cells |
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Preparation Method |
Cells were incubated with Humanin (1 uM) for 24 h. Cell viability was assessed by MTT reduction assay. |
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Reaction Conditions |
Humanin (1 uM) for 24 h |
|
Applications |
Humanin increased cell viability. HN decreased the percentage of hypodiploid KGN cells as well as the percentage of apoptotic KGN cells. |
| Animal experiment [2]: | |
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Animal models |
Nine-week-old male ICR mice |
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Preparation Method |
On the day of the test, the mice were intraperitoneally (i.p.) injected with a peptide solution (S14G- Humanin 200 nM, 100 µl/mouse) or vehicle (sterile endotoxin-free water) and returned to their home cage. |
|
Dosage form |
S14G- Humanin 200 nM, 100 µl/mouse |
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Applications |
Humanin may be involved in the response of HLECs to oxidative stress, and that Humanin expression was significantly upregulated under oxidative stress conditions. |
|
References: [1]. Marvaldi C, Martin D, et,al. Mitochondrial humanin peptide acts as a cytoprotective factor in granulosa cell survival. Reproduction. 2021 Apr 20;161(5):581-591. doi: 10.1530/REP-20-0197. PMID: 33764899. | |
| Cas No. | 330936-69-1 | SDF | |
| 别名 | 护脑素(人体) | ||
| 化学名 | L-methionyl-L-alanyl-L-prolyl-L-arginylglycyl-L-phenylalanyl-L-seryl-L-cysteinyl-L-leucyl-L-leucyl-L-leucyl-L-leucyl-L-threonyl-L-seryl-L-α-glutamyl-L-isoleucyl-L-α-aspartyl-L-leucyl-L-prolyl-L-valyl-L-lysyl-L-arginyl-L-arginyl-L-alanine | ||
| Canonical SMILES | N/A | ||
| 分子式 | C119H204N34O32S2 | 分子量 | 2687.2 |
| 溶解度 | DMSO : 100 mg/mL (37.21 mM; Need ultrasonic) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.3721 mL | 1.8607 mL | 3.7213 mL |
| 5 mM | 0.0744 mL | 0.3721 mL | 0.7443 mL |
| 10 mM | 0.0372 mL | 0.1861 mL | 0.3721 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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Quality Control & SDS
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- Purity: >98.00%
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