Humantenmine
(Synonyms: 葫蔓藤碱甲,Gelsenicine) 目录号 : GC39819Humantenmine 是可从中国线虫中分离出来的一种生物碱,有用于疼痛和风湿性关节炎的潜力。
Cas No.:82354-38-9
Sample solution is provided at 25 µL, 10mM.
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Humantenmine, a newalkaloid isolated from Gelsemium elegan Banth in China, has the potential for pain and rheumatic arthritis treatment[1][2].
[1]. Kun Yang, et al. Development and In-House Validation of a Sensitive LC-MS/MS Method for Simultaneous Quantification of Gelsemine, Koumine and Humantenmine in Porcine Plasma. J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Feb 15;1076:54-60. [2]. Rongjin Sun, et al. CYP3A4/5 Mediates the Metabolic Detoxification of Humantenmine, a Highly Toxic Alkaloid From Gelsemium Elegans Benth. J Appl Toxicol. 2019 Sep;39(9):1283-1292.
Cas No. | 82354-38-9 | SDF | |
别名 | 葫蔓藤碱甲,Gelsenicine | ||
Canonical SMILES | O=C1[C@]2([C@H](OC3)C[C@]4([H])[C@@]3([H])[C@@H](N=C4CC)C2)C5=CC=CC=C5N1OC | ||
分子式 | C19H22N2O3 | 分子量 | 326.39 |
溶解度 | DMSO : 100 mg/mL (306.38 mM; Need ultrasonic) | 储存条件 | 4°C, protect from light |
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1 mg | 5 mg | 10 mg | |
1 mM | 3.0638 mL | 15.3191 mL | 30.6382 mL |
5 mM | 0.6128 mL | 3.0638 mL | 6.1276 mL |
10 mM | 0.3064 mL | 1.5319 mL | 3.0638 mL |
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2.
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The Metabolism and Disposition of Koumine, Gelsemine and Humantenmine from Gelsemium
Curr Drug Metab 2019;20(7):583-591.PMID:31203797DOI:10.2174/1389200220666190614152304.
Background: Gelsemium is a toxic flowering plant of the Gelsemiaceae family. It is used to treat skin diseases in China, and it is an important medicinal and homeopathic plant in North America. Up to now, more than 200 compounds have been isolated and reported from Gelsemium. More than 120 of these are indole alkaloids, including the main components, koumine, gelsemine and Humantenmine which produce the pharmacological and toxicological effects of Gelsemium. However, their clinical application their limited by its narrow therapeutic window. Therefore, it is very important to study the metabolism and disposition of indole alkaloids from Gelsemium before their clinical application. This paper reviews all the reports on the metabolism and disposition of alkaloids isolated from Gelsemium at home and abroad. Methods: The metabolism and disposition of alkaloids from Gelsemium were searched by the Web of Science, NCBI, PubMed and some Chinese literature databases. Results: Only koumine, gelsemine and Humantenmine have been reported, and few other alkaloids have been described. These studies indicated that the three indole alkaloids are absorbed rapidly, widely distributed in tissues, extensively metabolized and rapidly eliminated. There are species differences in the metabolism of these alkaloids, which is the reason for the differences in their toxicity in animals and humans. Conclusion: This review not only explains the pharmacokinetics of indole alkaloids from Gelsemium but also facilitates further study on their metabolism and mechanism of toxicity.
CYP3A4/5 mediates the metabolic detoxification of Humantenmine, a highly toxic alkaloid from Gelsemium elegans Benth
J Appl Toxicol 2019 Sep;39(9):1283-1292.PMID:31119768DOI:10.1002/jat.3813.
Gelsemium elegans Benth., a well-known toxic herbal plant, is widely used to treat rheumatic arthritis, inflammation and other diseases. Gelsemium contains Humantenmine (HMT), which is an important bioactive and toxic alkaloid. Cytochrome P450 enzymes (CYPs) play important roles in the elimination and detoxification of exogenous substances. This study aimed to investigate the roles of CYPs in the metabolism and detoxification of HMT. First, metabolic studies were performed in vitro by using human liver microsomes, selective chemical inhibitors and recombinant human CYPs. Results indicated that four metabolites, including hydroxylation and oxidation metabolites, were found in human liver microsomes and identified based on their high-resolution mass spectrum. The isoform responsible for HMT metabolism was mainly CYP3A4/5. Second, the toxicity of HMT on L02 cells in the presence of the nicotinamide adenine dinucleotide phosphate system (NADPH) was significantly less than that without NADPH system. A CYP3A4/5 activity inhibition model was established by intraperitoneally injecting ketoconazole in mice and used to evaluate the role of CYP3A4/5 in HMT detoxification. In this model, the 14-day survival rate of the mice decreased to 17% after they were intragastrically treated with HMT, along with hepatic injury and increasing alanine aminotransferase (ALT) /aspartate aminotransferase (AST) levels. Overall, CYP3A4/5 mediated the metabolism and detoxification of HMT.
Development and in-house validation of a sensitive LC-MS/MS method for simultaneous quantification of gelsemine, koumine and Humantenmine in porcine plasma
J Chromatogr B Analyt Technol Biomed Life Sci 2018 Feb 15;1076:54-60.PMID:29406028DOI:10.1016/j.jchromb.2018.01.019.
Three monomers of G. elegans indole alkaloids (gelsemine, koumine and Humantenmine) were simultaneously detected in porcine plasma for the first time with the development and validation of a sensitive and reliable LC-ESI-MS/MS method. Using a gradient mobile phase at a constant flow rate of 0.2 mL/min via electrospray ionization (positive ion mode) in a multiple reaction monitoring (MRM) scan, gelsemine, koumine and Humantenmine were eluted, separated and detected at an appropriate retention time. The porcine plasma was prepared using protein precipitation with 1% formic acid-acetonitrile: methanol (2:1, v/v). Using matrix-matched calibration curves and weighted least squares linear regression, a good linearity (r2 > 0.99) was achieved with a concentration range of 0.1-200 μg/L for gelsemine, koumine and Humantenmine; estimated LOD and LOQ values were 0.10 μg/L and 0.2 μg/L, respectively. The mean of the recoveries was in the range of 82.68-100.35% of porcine plasma at four different levels, and the intra-day and inter-day precision (CV) were lower than 15% with a range of 2.46-8.76% and 2.73-10.83%, respectively. The proposed method has proved to be suitable for accurate, quantitative determination of gelsemine, koumine and Humantenmine in porcine plasma.
Development of a validated UPLC-MS/MS method for determination of Humantenmine in rat plasma and its application in pharmacokinetics and bioavailability studies
Biomed Chromatogr 2017 Dec;31(12).PMID:28557019DOI:10.1002/bmc.4017.
Humantenmine (HMT), the most toxic compound isolated from Gelsemium elegans Benth, is a well-known active herbal compound. A rapid and sensitive ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed and validated to estimate the absolute oral bioavailability of HMT in rats. Quantification was performed by multiple reaction monitoring using electrospray ionization operated in positive ion mode with transitions of m/z 327.14 → m/z 296.19 for HMT and m/z 323.20 → m/z 236.23 for gelsemine (internal standard, IS). The linear range of the calibration curve was 1-256 nmol/L, with a lower limit of quantification at 1 nmol/L. The accuracy of HMT ranged from 89.39 to 107.5%, and the precision was within 12.24% (RSD). Excellent recovery and negligible matrix effect were observed. HMT remained stable during storage, preparation and analytical procedures. The pharmacokinetics of HMT in rats showed that HMT reached the concentration peak at 12.50 ± 2.74 min with a peak concentration of 28.49 ± 6.65 nmol/L, and the corresponding area under the concentration-time curve (AUC0-t ) was 1142.42 ± 202.92 nmol/L min after 200 μg/kg HMT was orally administered to rats. The AUC0-t of HMT given at 20 μg/kg by tail vein administration was 1518.46 ± 192.24 nmol/L min. The calculated absolute bioavailability of HMT was 7.66%.
Two-Dimensional Liquid Chromatography Method for the Determination of Gelsemium Alkaloids in Honey
Foods 2022 Sep 17;11(18):2891.PMID:36141017DOI:10.3390/foods11182891.
Toxic Chinese medicine residues in honey pose a serious threat to consumer health. Gelsemium is one of the nine ancient poisons, making the whole plant virulent. The residue of Gelsemium alkaloid in honey causes poisoning from time to time. Therefore, it is very important to establish a method for the detection of Gelsemium alkaloids in honey. In this study, a method of solid phase extraction (SPE) with two-dimensional liquid chromatography (2D-LC) was developed for the first time for the simultaneous determination of Gelsemium alkaloids in honey, including gelsemine, koumine and Humantenmine. First, the honey samples were purified by a PRS cation exchange column and extracted with 5% ammoniated methanol. Then, we verified the methodological indicators, which were in line with the Codex Guideline requirements. The verification results are as follows: matrix-matched calibrations indicated that the correlation coefficients were higher than 0.998. The recovery was in the range of 81%-94.2% with an intraday precision (RSD) of ≤5.0% and interday RSD of ≤3.8%. The limit of detection for the three alkaloids was 2 ng/g. The limits of quantification for gelsemine and koumine were 5 ng/g, and Humantenmine was 20 ng/g. This method can be applied to the monitoring of Gelsemium alkaloids in honey.