Hygromycin B-d4
目录号 : GC49267
An internal standard for the quantification of hygromycin
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Hygromycin B-d4 is intended for use as an internal standard for the quantification of hygromycin B by GC- or LC-MS. Hygromycin B is an aminoglycoside antibiotic that has been found in S. hygroscopicus.1,2 It is active against E. coli (MIC = 150 µg/ml) and cytotoxic to both prokaryotic and eukaryotic cells via inhibition of protein synthesis.3,4,5 Hygromycin B has commonly been used in molecular and cell biology applications to select for transformed cells expressing the E. coli hygromycin resistance genes hyg or hph.4,5
1.Eustice, D.C., and Wilhelm, J.M.Mechanisms of action of aminoglycoside antibiotics in eucaryotic protein synthesisAntimicrob. Agents Chemother.26(1)53-60(1984) 2.CabaÑas, M.J., VÁzquez, D., and Modolell, J.Dual interference of hygromycin B with ribosomal translocation and with aminoacyl-tRNA recognitionEur. J. Biochem.8721-27(1978) 3.McGaha, S.M., and Champney, W.S.Hygromycin B inhibition of protein synthesis and ribosome biogenesis in Escherichia coliAntimicrob. Agents Chemother.51(2)591-596(2006) 4.Kaster, K.R., Burgett, S.G., Rao, R.N., et al.Analysis of a bacterial hygromycin B resistance gene by transcriptional and translational fusions and by DNA sequencingNucleic Acids Res.11(19)6895-6911(1983) 5.Blochlinger, K., and Diggelmann, H.Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eucaryotic cellsMol. Cell Biol.4(12)2929-2931(1984)
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 1.8815 mL | 9.4073 mL | 18.8147 mL |
| 5 mM | 0.3763 mL | 1.8815 mL | 3.7629 mL |
| 10 mM | 0.1881 mL | 0.9407 mL | 1.8815 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。