Home>>Signaling Pathways>> DNA Damage/DNA Repair>> DNA/RNA Synthesis>>Hygromycin B (hydrate)

Hygromycin B (hydrate) Sale

目录号 : GC48445

An aminoglycoside antibiotic

Hygromycin B (hydrate) Chemical Structure

规格 价格 库存 购买数量
500mg
¥1,028.00
现货
1g
¥1,422.00
现货

电话:400-920-5774 Email: sales@glpbio.cn

Customer Reviews

Based on customer reviews.

Sample solution is provided at 25 µL, 10mM.

Description

Hygromycin B is an antibiotic which is used in molecular and cell biology to select for transformed cells expressing the hygromycin resistance gene.1,2 It is an aminoglycoside produced by the bacterium S. hygroscopicus which kills prokaryotic and eukaryotic cells by inhibiting protein synthesis.3,4 The hygromycin resistance gene (hyg or hph) encodes a phosphotransferase which inhibits hygromycin through phosphorylation.2

1.Kaster, K.R., Burgett, S.G., Rao, R.N., et al.Analysis of a bacterial hygromycin B resistance gene by transcriptional and translational fusions and by DNA sequencingNucleic Acids Research11(19)6895-6911(1983) 2.Blochlinger, K., and Diggelmann, H.Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eucaryotic cellsMolecular and Cellular Biology4(12)2929-2931(1984) 3.Eustice, D.C., and Wilhelm, J.M.Mechanisms of action of aminoglycoside antibiotics in eucaryotic protein synthesisAntimicrobial Agents and Chemotherapy26(1)53-60(1984) 4.CabaÑas, M.J., VÁzquez, D., and Modolell, J.Dual interference of hygromycin B with ribosomal translocation and with aminoacyl-tRNA recognitionEuropean Journal of Biochemistry8721-27(1978)

化学性质

Cas No. SDF
Canonical SMILES O[C@H]1[C@@H](CO)O[C@@H](O[C@@]2([H])[C@H](O)[C@@H](NC)C[C@@H](N)[C@@H]2O)[C@]3([H])[C@@]1([H])OC4(O[C@@]([C@@H](N)CO)([H])[C@H](O)[C@H](O)[C@H]4O)O3.O
分子式 C20H37N3O13•XH2O 分子量 527.5
溶解度 DMSO: 3.3 mg/ml,PBS (pH 7.2): 3.3 mg/ml 储存条件 -20°C
General tips 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
Shipping Condition 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。

溶解性数据

制备储备液
1 mg 5 mg 10 mg
1 mM 1.8957 mL 9.4787 mL 18.9573 mL
5 mM 0.3791 mL 1.8957 mL 3.7915 mL
10 mM 0.1896 mL 0.9479 mL 1.8957 mL
  • 摩尔浓度计算器

  • 稀释计算器

  • 分子量计算器

质量
=
浓度
x
体积
x
分子量
 
 
 
*在配置溶液时,请务必参考产品标签上、MSDS / COA(可在Glpbio的产品页面获得)批次特异的分子量使用本工具。

计算

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % saline
计算重置

产品文档

Quality Control & SDS

View current batch: