IFN a 2a Human, Plant
目录号 : GP20384Interferon Alpha 2a Human Recombinant, Tobacco
Sample solution is provided at 25 µL, 10mM.
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| Purity | Greater than 99.0% as determined by both:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. | Source | Nicotiana Sp.Plant. |
| Phycical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | Shipped at Room temp. |
| Synonyms | Leukocyte interferon; B cell interferon; Type I interferon; IFNA2; IFN-a 2a. | ||
| Amino Acid Sequence | The sequence of the first twelve N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Gln-Thr-His-Ser-Leu-Gly-Ser-Arg. | ||
| Solubility | It is recommended to reconstitute the lyophilized Interferon-alpha 2a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
| Stability | Lyophilized Interferon alpha 2a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution IFN-alpha 2a should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. | ||
| Biological Activity | The specific activity was determined by a quantitative gene report bioassay using human Type I interferon-sensitive cells. Compared with bacterial derived recombinant IFN-a 2a, assayed in the same conditions, plant derived IFN-alpha 2a showed 6.1 б┴ 108 Units/mg in reference to a viral resistance assay using bovine kidney MDBK cells. | ||
| Formulation | Lyophilized, containing 50% SDS. | ||
IFN-alpha is produced by macrophages and has antiviral activities. Interferon stimulates the production of two enzymes: protein kinase and an oligoadenylate synthetase.
The specific activity was determined by a quantitative gene report bioassay using human Type I interferon-sensitive cells. Compared with bacterial derived recombinant IFN-a 2a, assayed in the same conditions, plant derived IFN-alpha 2a showed 6.1 б┴ 108 Units/mg in reference to a viral resistance assay using bovine kidney MDBK cells.
Lyophilized Interferon alpha 2a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution IFN-alpha 2a should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.