IFN g Rat
目录号 : GP20401IFN-Gamma Rat Recombinant
Sample solution is provided at 25 µL, 10mM.
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| Purity | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. | Source | Escherichia Coli. |
| Phycical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | Shipped at Room temp. |
| Synonyms | Immune IFN; type II IFN; T cell IFN; MAF; IFNG; IFG; IFI; IFN-gamma. | ||
| Amino Acid Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Gly-Tyr-Leu. | ||
| Solubility | It is recommended to reconstitute the lyophilized IFN-gamma in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
| Stability | Lyophilized IFN-gamma although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution IFN-gamma should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. | ||
| Biological Activity | The specific activity as determined by the cytopathic affect inhibition assay with murine L929 cells chalenged with EMC virus was < 0.1 ng/ml, corresponding to a specific activity of 10,000,000units/mg. | ||
| Formulation | Lyophilized from a 0.2ж╠m filtered concentrated (1mg/ml) solution in PBS, pH 7.4. | ||
IFN-gamma produced by lymphocytes activated by specific antigens or mitogens.IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I IFNs.
The specific activity as determined by the cytopathic affect inhibition assay with murine L929 cells chalenged with EMC virus was < 0.1 ng/ml, corresponding to a specific activity of 10,000,000units/mg.
Lyophilized IFN-gamma although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution IFN-gamma should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.