IGFBP3 Human
目录号 : GP20428IGFBP3 Human Recombinant
Sample solution is provided at 25 µL, 10mM.
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| Purity | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. | Source | Escherichia Coli. |
| Phycical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | Shipped at Room temp. |
| Synonyms | GH-dependant binding protein; IBP3; BP-53; IGFBP-3. | ||
| Amino Acid Sequence | GASSGGLGPV VRCEPCDARA LAQCAPPPAV CAELVREPGC GCCLTCALSE GQPCGIYTER CGSGLRCQPS PDEARPLQAL LDGRGLCVNA SAVSRLRAYL LPAPPAPGNA SESEEDRSAG EVESPSVSST HRVSDPKFHP LHSKIIIIKK GHAKDSQRYK VDYESQSTDT QNFSSESKRE TEYGPCRREM EDTLNHLKFL NVLSPRGVHI PNCDKKGFYK KKQCRPSKGR KRGFCWCVDK YGQPLPGYTT KGKEDVHCYS MQSK. | ||
| Solubility | It is recommended to reconstitute the lyophilizedIGFBP3 in sterile 20mM AcOH (acetic Acid) not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
| Stability | Lyophilized IBP3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution IGF-BP 3 should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. | ||
| Biological Activity | The ED50, calculated by by its ability to inhibit IGF-II induced proliferation of MCF-7 is< 0.2 µg/ml in the presence of 15 ng/ml of Human IGF-II, corresponding to a specific activity of 5,000,000units/mg. | ||
| Formulation | Lyophilized from a 0.2µm filtered concentrated (0.5mg/ml) solution in PBS, pH 7.4. | ||
IGFBP3 is a member of the IGFBP family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with IGFALS and either IGF I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
The ED50, calculated by by its ability to inhibit IGF-II induced proliferation of MCF-7 is< 0.2 µg/ml in the presence of 15 ng/ml of Human IGF-II, corresponding to a specific activity of 5,000,000units/mg.
Lyophilized IBP3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution IGF-BP 3 should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.