IgG Purity and Heterogeneity Assay Kit (IgG 纯度和异质性分析试剂盒)
目录号 : GK10032IgG Purity and Heterogeneity Assay Kit是一种基于十二烷基硫酸钠-毛细管凝胶电泳(CE-SDS)技术的IgG蛋白纯度和异质性测定试剂盒,适用于PA 800 Plus系统。
Sample solution is provided at 25 µL, 10mM.
IgG Purity and Heterogeneity Assay Kit is a IgG protein purity and heterogeneity assay kit based on sodium dodecyl sulfate-capillary gel electrophoresis (CE-SDS) technology, suitable for PA 800 Plus system.
IgG Purity and Heterogeneity Assay Kit can be used to detect the purity and heterogeneity of IgG molecules in reduced and non-reduced states. IgG (reduced and non-reduced) protein samples at specified concentrations were heat denatured in the presence of SDS and the denatured products were separated according to their molecular weight in capillaries containing replaceable gel matrices. The principle of this kit is similar to SDS-PAGE, but the advantage is that CE-SDS uses elastic quartz capillaries as separation channels and separates samples under the drive of a high-voltage DC electric field, which can effectively reduce the diffusion of components, resulting in sharp peaks and high separation efficiency.
IgG Purity and Heterogeneity Assay Kit can detect impurities as low as 0.1%.
IgG Purity and Heterogeneity Assay Kit should be stored at room temperature and should not be frozen.
IgG Purity and Heterogeneity Assay Kit是一种基于十二烷基硫酸钠-毛细管凝胶电泳(CE-SDS)技术的IgG蛋白纯度和异质性测定试剂盒,适用于PA 800 Plus系统。
IgG Purity and Heterogeneity Assay Kit能够用于检测还原和非还原状态的IgG分子的纯度和异质性。在SDS存在的情况下,指定浓度的 IgG(还原和非还原)蛋白样本发生热变性,根据分子量大小,变性产物在含有可更换凝胶基质的毛细管内进行分离。本试剂盒的原理与SDS-PAGE类似,但优势在于,CE-SDS是以弹性石英毛细管为分离通道,在高压直流电场的驱动下对样本进行分离,能够有效减少组分的扩散,所得峰型尖锐,分离效率高。
IgG Purity and Heterogeneity Assay Kit可检测出含量低至0.1%的杂质。
IgG Purity and Heterogeneity Assay Kit应储存在室温条件下,请勿冷冻。
Components | Quantity | |
1 | Acidic Wash Solution, 0.1 N HCl | 100mL |
2 | Basic Wash Solution, 0.1 N NaOH | 100mL |
3 | SDS-MW Gel Buffer, pH 7.2, 0.2% SDS | 140mL |
4 | SDS-MW Sample Buffer-100 mM Tris-HCl, pH 9.0, 1% SDS | 50mL |