IL 1 Alpha Rhesus Macaque
目录号 : GP20448Interleukin-1 Alpha Rhesus Macaque Recombinant
Sample solution is provided at 25 µL, 10mM.
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| Purity | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. | Source | Escherichia Coli. |
| Phycical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | Shipped at Room temp. |
| Synonyms | Hematopoietin-1; Lymphocyte-activating factor (LAF); Endogenous Pyrogen (EP); Leukocyte Endogenous Mediator (LEM); Mononuclear Cell Factor (MCF); IL-1 alpha;IL1; IL-1A; IL1F1. | ||
| Amino Acid Sequence | SAPFSFLSNM TYHFIRIIKH EFILNDTLNQ TIIRANDQHL TAAAIHNLDE AVKFDMGAYT SSKDDTKVPV ILRISKTQLY VSAQDEDQPV LLKEMPEINK TITGSETNFL FFWETHGTKN YFISVAHPNL FIATKHDNWV CLAKGLPSIT DFQILENQA | ||
| Solubility | It is recommended to reconstitute the lyophilized IL 1 alpha Recombinant in sterile distilled H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
| Stability | Lyophilized IL 1 alpha although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution IL 1 alpha should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. | ||
| Biological Activity | The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 10 pg/ml, corresponding to a specific activity of > 1.0×108IU/mg. | ||
| Formulation | The IL 1 alpha was lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4. | ||
IL-1 alpha is produced by activated macrophages, stimulates thymocyte proliferation by inducing il-2 release, b-cell maturation and proliferation, and fibroblast growth factor activity. IL1A proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 10 pg/ml, corresponding to a specific activity of > 1.0×108IU/mg.
Lyophilized IL 1 alpha although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution IL 1 alpha should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.