IL 3 Human, Sf9
目录号 : GP20489Interleukin-3 Human Recombinant, Sf9
Sample solution is provided at 25 µL, 10mM.
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| Purity | Greater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. | Source | Insect Cells. |
| Phycical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | Shipped at Room temp. |
| Synonyms | MCGF (Mast cell growth factor); Multi-CSF; HCGF; P-cell stimulation factor; IL-3; MGC79398; MGC79399. | ||
| Amino Acid Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Pro-Thr. | ||
| Solubility | It is recommended to reconstitute the lyophilized Interleukin-3 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
| Stability | Lyophilized Interleukin-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution IL3 should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. | ||
| Biological Activity | The ED50 as determined by the dose-dependant stimulation of TF-1 cells is < 0.1 ng/ml, corresponding to a Specific Activity of 10,000,000IU/mg. | ||
| Formulation | Lyophilized from a concentrated (1mg/ml) solution in water containing 0.5X PBS pH-7.4. | ||
IL3 is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
The ED50 as determined by the dose-dependant stimulation of TF-1 cells is < 0.1 ng/ml, corresponding to a Specific Activity of 10,000,000IU/mg.
Lyophilized Interleukin-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution IL3 should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.