IL 3 Mouse
目录号 : GP20490Interleukin-3 Mouse Recombinant
Sample solution is provided at 25 µL, 10mM.
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Interleukin-3 mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 135 amino acids and having a molecular mass of 15100 Dalton. The IL-3 is purified by proprietary chromatographic techniques.
| Purity | Greater than 97.0% as determined by:(a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE. | Source | Escherichia Coli. |
| Phycical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | Shipped at Room temp. |
| Synonyms | MCGF (Mast cell growth factor); Multi-CSF; HCGF; P-cell stimulation factor; IL-3; MGC79398; MGC79399. | ||
| Amino Acid Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Asp-Thr-His-Arg. | ||
| Solubility | It is recommended to reconstitute the lyophilized Interleukin-3 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
| Stability | Lyophilized Interleukin-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution IL-3 should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. | ||
| Biological Activity | The ED50 as determined by the dose-dependant stimulation of murine M-NFS-60 cells is < 0.05 ng/ml, corresponding to a Specific Activity of 20,000,000IU/mg. | ||
| Formulation | Lyophilized from a concentrated (1mg/ml) solution in water containing no additives. | ||
Interleukin-3 is a pleiotropic cytokine produced primarily by activated T cells. IL-3 is thought to function via specific cell surface receptors to stimulate the proliferation, differentiation and survival of haematopoietic cell lines. IL-3 has also been shown to affect the functional activity of a variety of other cell types including mast cells, eosinophils, megakaryocytes and basophils.
The ED50 as determined by the dose-dependant stimulation of murine M-NFS-60 cells is < 0.05 ng/ml, corresponding to a Specific Activity of 20,000,000IU/mg.
Lyophilized Interleukin-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution IL-3 should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.