IL 33 Rat
目录号 : GP20649Interleukin-33 Rat Recombinant
Sample solution is provided at 25 µL, 10mM.
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| Purity | Greater than 95.0% as determined by SDS-PAGE. | Source | Escherichia Coli. |
| Phycical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | Shipped at Room temp. |
| Synonyms | Interleukin 33; DVS27; NF-HEV; NKHEV; C9orf26; Interleukin-1 family member 11; IL- 1F11; Nuclear factor from high endothelial venules; NFEHEV; DKFZp586H0523; RP11-575C20.2; IL-33. | ||
| Amino Acid Sequence | SIQGTSLLTE SCALSTYNDQ SVSFVLENGC YVINVEDCGK NQEKDKVLLR YYESSFPAQS GDGVDGKKLM VNMSPIKDTD IWLNANDKDY SVELQKGDVS PPDQAFFVLH KKSSDFVSFE CKNLPGTYIG VKDNQLALVE ENDESCNNIM FKLSKM | ||
| Solubility | It is recommended to reconstitute the lyophilizedIL-33 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
| Stability | Lyophilized IL-33 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution IL-33 should be stored at 4°C between 2-7 days and for future use below -18°C .Please prevent freeze-thaw cycles. | ||
| Biological Activity | The ED50 was determined by the dose-dependent stimulation of the proliferation of murine D10S cells is <0.5ng/ml, corresponding to a specific activity of >2,000,000units/mg. | ||
| Formulation | Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4. | ||
Interleukin 33 (IL-33) is a 32kDa proinflammatory cytokine that may also regulate gene transcription in producer cells. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12kDa and C-terminal fragment of 18kDa by cleavage of caspase-1.
The ED50 was determined by the dose-dependent stimulation of the proliferation of murine D10S cells is <0.5ng/ml, corresponding to a specific activity of >2,000,000units/mg.
Lyophilized IL-33 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution IL-33 should be stored at 4°C between 2-7 days and for future use below -18°C .Please prevent freeze-thaw cycles.