IL 4 Mouse
目录号 : GP20500白介素 4 小鼠重组体
Sample solution is provided at 25 µL, 10mM.
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Interleukin-4 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 120 amino acids and having a molecular mass of 13500 Dalton. The IL-4 is purified by proprietary chromatographic techniques.
| Purity | Greater than 95.00% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. | Source | CHO |
| Phycical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | Shipped at Room temp. |
| Synonyms | BCGF; BCDF; B cell stimulating factor; BSF-1; Lymphocyte stimulatory factor 1; IL-4; MGC79402; Binetrakin; Pitrakinra. | ||
| Amino Acid Sequence | MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVCRASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNESKSTSLKDFLE SLKSIMQMDY S. | ||
| Solubility | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml | ||
| Stability | Lyophilized Interleukin-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution IL4 should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. | ||
| Biological Activity | The ED50 as determined by the dose-dependant induction of HT-2 cell proliferation is less than 2 ng/ml corresponding to a Specific Activity of 500,000IU/mg. | ||
| Formulation | Lyophilized from a 0.2µm concentrated (1mg/ml) solution in PBS pH 7.4. | ||
Interleukin-4 is a pleiotropic cytokine produced primarily by activated T lymphocytes, basophils and mast cells. Multiple immune response-modulating functions are performed by IL-4 on a variety of cell types and it has an important role in the regulator of isotype switching, induction of IgE production in B lymphocytes and differentiation of precursor T helper cells. IL-4 binds to both membrane-bound and secreted soluble IL-4 receptors.
The ED50 as determined by the dose-dependant induction of HT-2 cell proliferation is less than 2 ng/ml corresponding to a Specific Activity of 500,000IU/mg.
Lyophilized Interleukin-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution IL4 should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.