IL 7 Mouse
目录号 : GP20533白细胞介素 7 小鼠重组体
Sample solution is provided at 25 µL, 10mM.
Purity | Greater than 96.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. | Source | Escherichia Coli. |
Phycical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | Shipped at Room temp. |
Synonyms | Lymphopoietin 1 (LP-1); pre-B cell factor; IL-7. | ||
Amino Acid Sequence | ECHIKDKEGK AYESVLMISI DELDKMTGTD SNCPNNEPNF FRKHVCDDTK EAAFLNRAAR KLKQFLKMNI SEEFNVHLLT VSQGTQTLVN CTSKEEKNVK EQKKNDACFL KRLLREIKTC WNKILKGSI. | ||
Solubility | It is recommended to reconstitute the lyophilized Interleukin 7 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
Stability | Lyophilized Interleukin-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution IL7 should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. | ||
Biological Activity | The ED50was determined by the dose-dependent stimulation of the proliferation of murine 2E8 cells is less than 0.2 ng/ml, corresponding to a specific activity of >5.0б┴106 IU/mg. | ||
Formulation | Filtered (0.2µm) and lyophilized from a concentrated (1mg/ml) solution in 1б┴PBS, pH7.4 and 2% trehalose. |
Interleukin-7 is a potent lymphoid cell growth factor produced primarily by stromal cells. IL-7 has been shown to support the proliferation and differentiation of pre B- and early T cells as well as displaying a biological effect on cells of NK and myeloid lineages.
The ED50was determined by the dose-dependent stimulation of the proliferation of murine 2E8 cells is less than 0.2 ng/ml, corresponding to a specific activity of >5.0б┴106 IU/mg.
Lyophilized Interleukin-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution IL7 should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.