IL 8 Porcine
目录号 : GP21128Interleukin-8 (1-72 a.a) Porcine Recombinant (CXCL8)
Sample solution is provided at 25 µL, 10mM.
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| Purity | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. | Source | Escherichia Coli. |
| Phycical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | Shipped at Room temp. |
| Synonyms | IL-8; CXCL8; Monocyte-derived neutrophil chemotactic factor; MDNCF; T-cell chemotactic factor; Neutrophil-activating protein 1; NAP-1; Protein 3-10C; Granulocyte chemotactic protein 1; GCP-1; Monocyte-derived neutrophil-activating peptide; MONAP; Emoctakin; K60; NAF; LECT; LUCT; 3-10C; LYNAP; SCYB8; TSG-1; AMCF-I; b-ENAP; Alveolar macrophage chemotactic factor I. | ||
| Amino Acid Sequence | ARVSAELRCQ CINTHSTPFH PKFIKELRVI ESGPHCENSE IIVKLVNGKE VCLDPKEKWV QKVVQIFLKR TEKQQQQQ. | ||
| Solubility | It is recommended to reconstitute the lyophilized Interleukin 8 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
| Stability | Lyophilized Interleukin-8 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution CXCL8 should be stored at 4°C between 2-7 days and for future use below -18°C . For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. | ||
| Biological Activity | The biological activity was determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration range of 20-200 ng/ml. | ||
| Formulation | Lyophilized from a 0.2µm filtered concentrated solution in 1б┴PBS, pH 7.4. | ||
Interleukin-8 (IL-8) is a chemokine produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles, the Weibel-Palade bodies. When first encountering an antigen, the primary cells to encounter it are the macrophages who phagocytose the particle. Upon processing, they release chemokines to signal other immune cells to come in to the site of inflammation. IL-8 is one such chemokine. It serves as a chemical signal that attracts neutrophils at the site of inflammation, and therefore is also known as Neutrophil Chemotactic Factor.
The biological activity was determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration range of 20-200 ng/ml.
Lyophilized Interleukin-8 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution CXCL8 should be stored at 4°C between 2-7 days and for future use below -18°C . For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.