IM-93
目录号 : GC45793A dual inhibitor of ferroptosis and NETosis
Cas No.:1173657-73-2
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
IM-93 is a dual inhibitor of ferroptosis and NETosis.1 It inhibits tert-butyl hydroperoxide- and erastin-induced ferroptosis in NIH3T3 cells (IC50s =1.8 and 1.9 nM, respectively), as well as decreases NETosis induced by phorbol 12-myristate 13-acetate in isolated human peripheral blood neutrophils when used at concentrations ranging from 1.6 to 25 μM. IM-93 also inhibits hydrogen peroxide-induced necrosis in HL-60 cells (IC50 = 0.45 μM), but has no effect on necroptosis induced by Fas ligand in combination with Z-VAD-FMK and cycloheximide in Jurkat cells or pyroptosis induced by S. aureus and P. aeruginosa in THP-1 cells when used at a concentration of 25 μM.
|1. Dodo, K., Kuboki, E., Shimizu, T., et al. Development of a water-soluble indolylmaleimide derivative IM-93 showing dual inhibition of ferroptosis and NETosis. ACS Med. Chem. Lett. 10(9), 1272-1278 (2019).
Cas No. | 1173657-73-2 | SDF | |
Canonical SMILES | O=C(C(C1=CN(C)C2=C1C=CC=C2)=C3NCCCN(C)C)N(C(C)C)C3=O | ||
分子式 | C21H28N4O2 | 分子量 | 368.5 |
溶解度 | DMF: 20mg/mL,DMSO: 20mg/mL,DMSO:PBS (pH 7.2) (1:2): 0.3mg/mL,Ethanol: 1mg/mL | 储存条件 | Store at -20°C |
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制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 2.7137 mL | 13.5685 mL | 27.137 mL |
5 mM | 0.5427 mL | 2.7137 mL | 5.4274 mL |
10 mM | 0.2714 mL | 1.3569 mL | 2.7137 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
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% DMSO % % Tween 80 % saline | ||||||||||
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工作液浓度: mg/ml;
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1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
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Development of a Water-Soluble Indolylmaleimide Derivative IM-93 Showing Dual Inhibition of Ferroptosis and NETosis
ACS Med Chem Lett 2019 Jul 30;10(9):1272-1278.PMID:31531196DOI:PMC6746101
The indolylmaleimide (IM) derivative IM-17 shows inhibitory activity against oxidative-stress-induced necrotic cell death and cardioprotective activity in rat ischemia-reperfusion injury models. In order to develop a more potent derivative, we conducted a detailed structure-activity relationship study of IM derivatives and identified IM-93 as the most potent derivative with good water solubility. IM-93 inhibited ferroptosis and NETosis, but not necroptosis or pyroptosis. In contrast, ferrostatin-1 (Fer-1), a ferroptosis inhibitor, did not inhibit NETosis, although the accompanying lipid peroxidation was partially inhibited by Fer-1, as well as by IM-93. Thus, IM derivatives have a unique activity profile and appear to be promising candidates for in vivo application.