Imisopasem manganese (M40403)
(Synonyms: M40403; GC4403) 目录号 : GC34104
A SOD mimetic
Cas No.:218791-21-0
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
Imisopasem manganese is a nonpeptide superoxide dismutase (SOD) mimetic.1 It catalyzes dismutation of the superoxide anion with a catalytic rate constant of greater than 2 x 107 M-1s-1. Imisopasem manganese (1-10 mg/kg, i.v. bolus) reduces carrageenan-induced paw edema and inhibits increases in paw exudate levels of TNF-α, IL-1β, and lactate dehydrogenase (LDH) in rats. It inhibits increases in lung and ileum myeloperoxidase (MPO) levels, indicating reduced neutrophil infiltration, and increases in plasma malondialdehyde (MDA), TNF-α, and IL-1β levels in a rat model of ischemia-reperfusion injury and shock induced by splanchnic artery occlusion (SAO) when administered at doses of 0.1, 0.3, and 1 mg/kg.
1.Salvemini, D., Wang, Z.Q., Zweier, J.L., et al.A nonpeptidyl mimic of superoxide dismutase with therapeutic activity in ratsScience286(5438)304-306(1999)
|
Animal experiment: |
Rats: Male Sprague-Dawley rats are used in the study. M40403 (5-20 mg kg-1), or an equivalent volume (0.3 mL) of vehicle (26 mm sodium bicarbonate buffer, pH 8.1-8.3), is injected intraperitoneally (i.p.) 15 min before carrageenan. At 4 h after the injection of carrageenan, the animals are killed by inhalation of CO2[1]. Mice: 30 mg dry powder M40403 is dissolved in 6.0 ml SBC adjusted to pH 8.3 with 1 M NaOH. This stock solution is diluted to 1.25 mg/mL in SBC. Two experimental models are tested. In one, the dose of IR is held constant at 8.5 Gy total body irradiation (TBI). The mice are injected i.p. with a single dose of 40 mg/kg, 30 mg/kg, 20 mg/kg or 10 mg/kg M40403. Thirty minutes later the mice receives 8.5 Gy total body irradiation (TBI). Control animals receives 0.1 ml of SBC buffer prior to TBI. In the other model, groups of 20 mice receives either 6.5 or 7.5 Gy TBI. One half of each group is treated with 2.0 mg/kg M40403 i.p. and the other with SBC 30 min before TBI. All are followed for survival[2]. |
|
References: [1]. Salvemini D, et al. Pharmacological manipulation of the inflammatory cascade by the superoxide dismutase mimetic,M40403. Br J Pharmacol. 2001 Feb;132(4):815-27. |
|
| Cas No. | 218791-21-0 | SDF | |
| 别名 | M40403; GC4403 | ||
| Canonical SMILES | [Cl-][Mn]1234([Cl-])([Mn+])[N]5=C6C=CC=C5C[NH]1[C@]7([H])CCCC[C@@]7([H])[NH]2CC[NH]3[C@@](CCCC8)([H])[C@]8([H])[NH]4C6 | ||
| 分子式 | C21H35Cl2Mn2N5- | 分子量 | 538.32 |
| 溶解度 | Water : 16 mg/mL (29.72 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 1.8576 mL | 9.2882 mL | 18.5763 mL |
| 5 mM | 0.3715 mL | 1.8576 mL | 3.7153 mL |
| 10 mM | 0.1858 mL | 0.9288 mL | 1.8576 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: ≥96.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet