Imperatorin
(Synonyms: 欧前胡素; Ammidin) 目录号 : GN10437
A furanocoumarin with diverse biological activities
Cas No.:482-44-0
Sample solution is provided at 25 µL, 10mM.
Imperatorin is reported as an anti-cancer medicine with many targets to induce cell apoptosis, such as enhancing p53 expression [1], inducing Mcl-1 degradation [2], activating activation of caspase-3, caspase-8, caspase-9 [3].
When tested with human cancer cell lines (SNU 449-liver cancer and HCT-15-colon cancer), imperatorin treatment promoted cell apoptosi in a dose-dependent manner via modulating expression of apotosis-related proteins [4]. In human embryonic kidney cells, imperatorin treatment significantly attenuated H2O2-induced ROS production [5]. When tested with human hepatoma HepG2 cells, administration of imperatorin inhibited cell proliferation in a time- and dose- dependent manner [3]. In human lung cancer H23 cells, imperatorin was shown to enhance the expression of p53 and induced cells apoptosis at sub-toxic concentrations [1].
In nude mouse model bearing HepG2 cells, treatment with imperatorin significantly suppressed tumor growth in a dose-dependent manner [3].
It is also reported that imperatorin inhibits voltage-gated K+ channels and ATP-sensitive K+ channels when tested with neuronal NG-108-15 cells [6].
References:
[1]. Choochuay, K., et al., Imperatorin sensitizes anoikis and inhibits anchorage-independent growth of lung cancer cells. J Nat Med, 2013. 67(3): p. 599-606.
[2]. Li, X., et al., Imperatorin induces Mcl-1 degradation to cooperatively trigger Bax translocation and Bak activation to suppress drug-resistant human hepatoma. Cancer Lett, 2014. 348(1-2): p. 146-55.
[3]. Luo, K.W., et al., Anticancer effects of imperatorin isolated from Angelica dahurica: induction of apoptosis in HepG2 cells through both death-receptor- and mitochondria-mediated pathways. Chemotherapy, 2011. 57(6): p. 449-59.
[4]. Rahman, A., et al., Growth inhibition of various human cancer cell lines by imperatorin and limonin from poncirus trifoliata rafin. Seeds. Anticancer Agents Med Chem, 2015. 15(2): p. 236-41.
[5]. Cao, Y., et al., Antioxidant effect of imperatorin from Angelica dahurica in hypertension via inhibiting NADPH oxidase activation and MAPK pathway. J Am Soc Hypertens, 2014. 8(8): p. 527-36.
[6]. Wang, Y.W., et al., Inhibitory effects of imperatorin on voltage-gated K(+) channels and ATP-sensitive K(+) channels. Pharmacol Rep, 2015. 67(1): p. 134-9.
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Animal experiment: |
Mice[3]The experiments are carried out on naive male Swiss mice weighing 20-25 g. Drugs are administered intraperitoneally (i.p.) at the volume of 10 mL/kg. Fresh drug solutions are prepared on each day of experimentation. Control groups receive saline injections of the same volume and via the same route of administration. During the acute treatment, the animals are allocated to the following drug groups: saline, rivastigmine (0.5 mg/kg, i.p.), scopolamine (1 mg/kg, i.p.), Imperatorin (1, 5, 10 mg/kg, i.p.), or Imperatorin coadministered with scopolamine. To measure the memory acquisition processes, scopolamine is administered 20 min before the pretest, whereas Imperatorin and rivastigmine are administered 30 min before the pretest. To measure the memory consolidation processes, rivastigmine or scopolamine (1 mg/kg) is administered immediately after the pretest, whereas Imperatorin is administered 15 min after pretest or after scopolamine injection. On the second day, the mice are retested. In the second set of the experiments, animals are randomly allocated to receive 6 days of i.p. injections of Imperatorin (1, 5, and 10 mg/kg, i.p.) or saline, twice daily (8:00 a.m. and 8:00 p.m.). On the seventh day, these animals are subjected to saline, scopolamine (1 mg/kg, i.p.), Imperatorin (1, 5, and 10 mg/kg, i.p.), or Imperatorin coadministered with scopolamine. To measure the memory acquisition processes, scopolamine is administered 20 min before the pretest and Imperatorin 30 min before the pretest. To measure the memory consolidation processes, scopolamine (1 mg/kg) is administered immediately after the pretest, whereas Imperatorin is administered 15 min after the pretest or after scopolamine injection. On the eighth day, the mice are retested. |
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References: [1]. Kozioł E, et al. Imperatorin-pharmacological meaning and analytical clues: profound investigation. Phytochem Rev. 2016;15:627-649. |
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| Cas No. | 482-44-0 | SDF | |
| 别名 | 欧前胡素; Ammidin | ||
| 化学名 | 9-(3-methylbut-2-enoxy)furo[3,2-g]chromen-7-one | ||
| Canonical SMILES | CC(=CCOC1=C2C(=CC3=C1OC=C3)C=CC(=O)O2)C | ||
| 分子式 | C16H14O4 | 分子量 | 270.28 |
| 溶解度 | DMF: 30 mg/mL,DMF:PBS (pH 7.2)(1:3): 0.25 mg/mL,DMSO: 20 mg/mL,Ethanol: 10 mg/mL | 储存条件 | Store at 2-8°C,protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.6999 mL | 18.4993 mL | 36.9987 mL |
| 5 mM | 0.74 mL | 3.6999 mL | 7.3997 mL |
| 10 mM | 0.37 mL | 1.8499 mL | 3.6999 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >98.00%
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