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Indirubin Sale

(Synonyms: 靛玉红; Couroupitine B; Indigo red; Indigopurpurin) 目录号 : GC12975

A natural inhibitor of GSK3 and CDK isoforms

Indirubin Chemical Structure

Cas No.:479-41-4

规格 价格 库存 购买数量
10mM (in 1mL DMSO)
¥266.00
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5mg
¥226.00
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25mg
¥1,080.00
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100mg
¥2,555.00
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250mg
¥4,103.00
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Sample solution is provided at 25 µL, 10mM.

产品文档

Quality Control & SDS

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实验参考方法

Cell experiment [1]:

Cell lines

HBL-38 and HPB-ALL cells

Preparation method

The solubility of this compound in DMSO is > 10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months.

Reacting condition

0 ~ 1000 ng/mL; for 24 hrs

Applications

In HBL-38 cells, Indirubin (10 ng/mL) strongly inhibited interferon-γ production without any influence on cell proliferation. The IC50 value of Indirubin was 0.15 ng/mL. However, in HPB-ALL cells, Indirubin did not affect interleukin-4 production stimulated by phytohemaggulutinin, as well as cell proliferation.

Animal experiment [1]:

Animal models

TNCB-induced delayed-type hypersensitivity mouse model

Dosage form

20 mg/day; i.p.; 4 days before sensitization to 4 days after sensitization

Applications

In the single elicitation experiment, Indirubin significantly inhibited ear swelling of the TNCB-induced delayed-type hypersensitivity mouse model at the 24th hr after treatment. In the experiment with repeated epicutaneous applications, Indirubin also inhibited ear swelling but these effects gradually reduced. Meanwhile, Indirubin did not cause any body weight loss.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1]. Kunikata T, Tatefuji T, Aga H, Iwaki K, Ikeda M, Kurimoto M. Indirubin inhibits inflammatory reactions in delayed-type hypersensitivity. Eur J Pharmacol. 2000 Dec 20;410(1):93-100.

产品描述

Interferon-γ is thought to be a member of the inflammatory cytokine family. Because of its augmentation of inflammatory reactions through biological activities. Indirubin, the active ingredient of Danggui Longhui Wan, has inhibitory effects on the production of interferon-γ.

In vitro: Indirubin exerted its inhibitory effects not only on interferon-γ production by human myelomonocytic HBL-38 cells but also on interferon-γ and interleukin-σ production by murine splenocytes with no influence on the proliferation of either cells [1].

In vivo: Because of Indirubin’s inhibitory activity on interferon-γ production, the authors further investigated the effects of indirubin on 2,4,6-trinitro-l-chlorobenzene-induced delayed-type hypersensitivity. When injected intraperitoneally, indirubin significantly inhibited the ear swelling of TNCB-elicited mice. Moreover,the amount of interferon-γ in the culture supernatants of elicited mouse lymphocytes was inhibited by indirubin treatment [1].

Clinical trials: Several clnical trials have been conducted to study the efficacy of indirubin for the treatments of psoriasis and Acute Promyeloid Leukemia .

Reference:
[1] Kunikata T, Tatefuji T, Aga H, Iwaki K, Ikeda M, Kurimoto M.   Indirubin inhibits inflammatory reactions in delayed-type hypersensitivity. Eur J Pharmacol. 2000 Dec 20;410(1):93-100.

Chemical Properties

Cas No. 479-41-4 SDF
别名 靛玉红; Couroupitine B; Indigo red; Indigopurpurin
Canonical SMILES O=C1/C(NC2=CC=CC=C21)=C3C4=CC=CC=C4NC\3=O
分子式 C16H10N2O2 分子量 262.26
溶解度 DMF>1 mg/ml, DMSO>10 mg/ml 储存条件 Store at -20°C
General tips 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
Shipping Condition 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。

溶解性数据

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1 mg 5 mg 10 mg
1 mM 3.813 mL 19.065 mL 38.1301 mL
5 mM 0.7626 mL 3.813 mL 7.626 mL
10 mM 0.3813 mL 1.9065 mL 3.813 mL
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