Insulin Human, Yeast
目录号 : GP20674Insulin Human Recombinant, Yeast
Sample solution is provided at 25 µL, 10mM.
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Insulin Human Recombinant produced in Yeast is a two chain, glycosylated polypeptide chain containing 51 amino acids and having a molecular mass of 5807 Dalton. Zinc content was found to be 0.4%. Insulin is purified by proprietary chromatographic techniques.
| Purity | Greater than 95.0% as determined by SDS-PAGE. | Source | Saccharomyces cerevisiae. |
| Phycical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | Shipped at Room temp. |
| Solubility | It is recommended to reconstitute the lyophilized Insulin in PBS pH-7.5. | ||
| Stability | Lyophilized Insulin although stable at room temperature, should be stored at 4°C . Upon reconstitution Insulin should be stored at -20°C to -80°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. | ||
| Biological Activity | 27.5 units/mg. Insulin has been evaluated in cell culture (Human Foreskin Fibroblasts). The effective concentration range for use in defined media is 1-5ug/ml. However, the optimum concentration range for a particular application must be determined by the investigator. | ||
| Formulation | The protein was lyophilized from 50mM Sodium Chloride solution. | ||
Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
27.5 units/mg. Insulin has been evaluated in cell culture (Human Foreskin Fibroblasts). The effective concentration range for use in defined media is 1-5ug/ml. However, the optimum concentration range for a particular application must be determined by the investigator.
Lyophilized Insulin although stable at room temperature, should be stored at 4°C . Upon reconstitution Insulin should be stored at -20°C to -80°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.