Iodoacetyl-LC-Biotin
(Synonyms: N-碘代乙酰基-N'-生物素基-1,6-己二胺,N-Biotinyl-N'-(iodoacetyl)-1,6-hexanediamine,Iaa-biotin) 目录号 : GC11968
碘乙酰基-LC-生物素是一种生物素化的亲电子探针，可用于研究蛋白质与亚细胞蛋白质组共价结合的范围和特征。
Cas No.:93285-75-7
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
| Biotinylation method [1]: | |
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Sample |
Immunoglobulin G |
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Preparation method |
Soluble in DMSO or DMF. |
|
Reaction Conditions |
4mM, room temperature for 90min |
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Applications |
Immunoglobulin G (26.7 nmol) was dissolved in 1ml of 0.1M sodium phosphate, 5Mm EDTA buffer, pH 6. To this solution, DTT was added to produce a 50mM final concentration and incubation for 90min at 37 °C, the solution was allowed to cool to room temperature. Excess DTT was removed by molecular sieve chromatography using 50mM Tris, pH 8.3, with 5mM EDTA as the elution buffer. The IgG with reduced sulfhydryl groups was then biotinylated; 30 μl of 4mM iodoacetyl-LC-biotin in Me2SO was added and the mixture was incubated for 90min at room temperature in the dark. Finally, residual biotinylating reagent and free biotin were removed by dialysis against 0.1mM PBS, pH 7.2, for 72h. Protein concentrations were determined by BCA protein assay. Total biotin covalently bound to IgG was determined by an avidin-binding assay. |
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References: [1]. Anna Bogusiewicz,Nell I. Mock, and Donald M. Mock. Instability of the biotin–protein bond in human plasma. Analytical Biochemistry 327 (2004) 156–161. | |
Iodoacetyl-LC-Biotin (N-iodoacetyl-N-biotinylhexylenediamine), a sulfhydryl-reactive biotinylation agent, is a water-insoluble reagent that requires the dissolution of suitable solvents, including dimethyl sulfoxide (DMSO) and dimethylformamide (DMF), prior to the addition into aqueous reactions. Iodoacetyl-LC-Biotin consists of a bicyclic biotin rings structure, a 1,6-diaminohexane spacer group attached to the valeric acid side chain of biotin and an iodoacetyl group at the end of the spacer providing sulfhydryl reactivity. The iodoacetyl group at the end of Iodoacetyl-LC-Biotin is able to react with sulfhydryl groups on proteins and other molecules forming a nonreversible bond. The long spacer arm of Iodoacetyl-LC-Biotin enables the modified molecule to better bind to the avidin or streptavidin probes.
Reference
[1].Bioconjugate Techniques , 2nd ed. By Greg T.Hermanson (Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL). Academic Press (an imprint of Elsevier): London, Amsterdam, Burlington, San Diego . 2008. ISBN 978-0-12-370501-3.
| Cas No. | 93285-75-7 | SDF | |
| 别名 | N-碘代乙酰基-N'-生物素基-1,6-己二胺,N-Biotinyl-N'-(iodoacetyl)-1,6-hexanediamine,Iaa-biotin | ||
| 化学名 | 5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-N-[6-[(2-iodoacetyl)amino]hexyl]pentanamide | ||
| Canonical SMILES | C1C2C(C(S1)CCCCC(=O)NCCCCCCNC(=O)CI)NC(=O)N2 | ||
| 分子式 | C18H31IN4O3S | 分子量 | 510.43 |
| 溶解度 | ≥ 51mg/mL in DMSO with gentle warming | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.9591 mL | 9.7957 mL | 19.5913 mL |
| 5 mM | 0.3918 mL | 1.9591 mL | 3.9183 mL |
| 10 mM | 0.1959 mL | 0.9796 mL | 1.9591 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。