Ionomycin calcium salt
(Synonyms: 罗红霉素钙盐(链霉菌属载体),SQ23377 calcium) 目录号 : GC15148Ionomycin calcium salt是一种窄谱抗生素,对革兰氏阳性菌具有活性,由丛生链霉菌(Streptomyces conglobatus)产生。
Cas No.:56092-82-1
Sample solution is provided at 25 µL, 10mM.
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Ionomycin calcium salt is a narrow spectrum antibiotic being active against Gram-positive bacteria, which produced by the bacterium Streptomyces conglobatus[1]. Ionomycin calcium salt promotes apoptosis. Ionomycin also induces the activation of protein kinase C (PKC)[2].
In vitro, treatment with 2 µM Ionomycin in LCLC 103H cells results in an instantaneous increase in intracellular Ca2+ concentration from 50 to 180 nM. And in Ionomycin-treated cultures, there is obvious DNA fragmentation and PARP cleavage to an 85-kDa fragment, and necrosis could be detected in ~1-5% of the Ionomycin treated cells[2]. In ras oncogene expressing cells, Ionomycin leads to a dose dependent increase of intracellular calcium activity. At 100 nmol/l ionomycin intracellular calcium is increased from 114 ± 17 nmol/l to 342 ± 24 nmol/l, a value within the range of intracellular calcium concentrations following application of bradykinin[3].
In vivo efficacy test, treatment with 1 µm ionomycin for 6 h, ubiquitination of ENaC-β was robustly enhanced in cells overexpressing wild-type Nedd4-2a[4].
References:
[1] Liu WC, et al. Ionomycin, a new polyether antibiotic. J Antibiot (Tokyo). 1978 Sep;31(9):815-9.
[2] Chatila T, et al. Mechanisms of T cell activation by the calcium ionophore ionomycin. J Immunol. 1989 Aug 15;143(4):1283-9.
[3] WÖll E, et al. The role of calcium in cell shrinkage and intracellular alkalinization by bradykinin in Ha-ras oncogene expressing cells. FEBS Lett. 1993 May 17;322(3):261-5.
[4] Wang J, et al. Calcium activates Nedd4 E3 ubiquitin ligases by releasing the C2 domain-mediated auto-inhibition. J Biol Chem. 2010 Apr 16;285(16):12279-88.
Ionomycin calcium salt是一种窄谱抗生素,对革兰氏阳性菌具有活性,由丛生链霉菌(Streptomyces conglobatus)产生[1]。Ionomycin calcium salt可促进细胞凋亡。Ionomycin calcium salt还能诱导激活蛋白激酶 C (PKC)[2]。
在体外,用 2 µM Ionomycin calcium sal处理 LCLC 103H 细胞,细胞内 Ca2+ 浓度会瞬间从 50 nM 增至 180 nM。在经Ionomycin calcium sal处理的培养物中,会出现明显的 DNA 断裂和 PARP 分裂,形成 85 kDa 的片段,在约 1-5% 经Ionomycin calcium sal处理的细胞中可检测到坏死[2]。在表达ras癌基因的细胞中,Ionomycin calcium sal会导致细胞内钙活性的剂量依赖性增加。当Ionomycin calcium sal浓度为 100 nmol/l 时,细胞内钙的浓度从 114 ± 17 nmol/l 增至 342 ± 24 nmol/l,这一数值在使用缓激肽后细胞内钙浓度的范围内[3]。
在体内药效试验中,用 1 µm Ionomycin calcium sal处理 6 小时后,在过表达野生型 Nedd4-2a 的细胞中,ENaC-β 的泛素化显著增强[4]。
| Cell experiment [1]: | |
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Cell lines |
N1E-115 cells |
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Preparation Method |
Cells were treated with various concentrations (0, 0.2, 0.5, 1, 2, and 10 µM) and incubation times (3, 6, 12, and 24 h) of ionomycin, and cell survival was determined using the trypan blue dye exclusion assay. |
|
Reaction Conditions |
0, 0.2, 0.5, 1, 2, and 10 µM；3, 6, 12, and 24 h |
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Applications |
Ionomycin treatment induced cell death in a concentration- and time-dependent manner. |
| Animal experiment [2]: | |
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Animal models |
Adult male C57BL/6 wild type (20-25 g, 8-10 weeks old) and FXR KO mice (20-25 g, 8-10 weeks old) |
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Preparation Method |
In order to investigate the biological function of FXR in vivo, an L-type calcium channel agonist, Bayk8644 (2 mg/kg) was given intraperitoneally at 10 min after reperfusion. Bayk8644 stock solution (1 mmol/L in dimethyl sulfoxide, DMSO) was diluted in 1× phosphate-buffered saline (PBS) with a final DMSO concentration of 4.6%. Similarly, PBS with 4.6% DMSO was given intraperitoneally in sham, wild-type vehicle, and FXR knockout vehicle groups as control. A dose range of ionomycin (50 nmol/L), GW4064 (0, 1, 1.5, 3, and 6 µmol/L), or Z-guggulsterone (Z-GS, 0, 2.5, 5, and 10 µmol/L) was given for 18 h before oxygen and glucose deprivation (OGD). |
|
Dosage form |
50 nmol/L (4.6% DMSO); 18 h; i.p. |
|
Applications |
TUNEL staining demonstrated that Z-GS treatment reduced neuronal apoptosis and addition of IM (Ionomycin) significantly increased apoptotic neurons compared to OGD group, suggesting that inhibition of FXR (Farnesoid X receptor) can reduce neuronal apoptosis through reducing calcium influx after OGD/R. |
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References: Nakamura S, et al. Ionomycin-induced calcium influx induces neurite degeneration in mouse neuroblastoma cells: analysis of a time-lapse live cell imaging system. Free Radic Res. 2016;50(11):1214-1225. |
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| Cas No. | 56092-82-1 | SDF | |
| 别名 | 罗红霉素钙盐(链霉菌属载体),SQ23377 calcium | ||
| 化学名 | calcium (4S,6S,8S,10E,12R,14R,16E,18S,19S,20R,21S)-19,21-dihydroxy-22-((2R,2'R,5R,5'S)-5'-((R)-1-hydroxyethyl)-2,5'-dimethyloctahydro-[2,2'-bifuran]-5-yl)-4,6,8,12,14,18,20-heptamethyl-11-oxido-9-oxodocosa-10,16-dienoate | ||
| Canonical SMILES | O[C@H](C)[C@@]1(C)O[C@H](CC1)[C@]2(C)O[C@@H](C[C@@H]([C@@H](C)[C@H]([C@H](/C=C/C[C@@H](C)C[C@@H](C)/C([O-])=C\C([C@@H](C)C[C@@H](C)C[C@@H](C)CCC([O-])=O)=O)C)O)O)CC2.[Ca+2] | ||
| 分子式 | C41H70O9.Ca | 分子量 | 747.08 |
| 溶解度 | 20mg/mL in enathol, 1.6mg/mL in DMSO, 1.2mg/mL in DMF | 储存条件 | Store at -20°C,protect from light |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.3385 mL | 6.6927 mL | 13.3854 mL |
| 5 mM | 0.2677 mL | 1.3385 mL | 2.6771 mL |
| 10 mM | 0.1339 mL | 0.6693 mL | 1.3385 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
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