IR 780
(Synonyms: IR-780碘化物) 目录号 : GC48396
IR 780 是一种七次甲基花青荧光探针,用于肿瘤细胞的体内成像,吸收光谱750-830nm。
Cas No.:207399-07-3
Sample solution is provided at 25 µL, 10mM.
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IR 780 is a heptacyanine fluorescent probe for in vivo imaging of tumor cells with a large absorption spectrum of 750-830nm.It is also a powerful and stable nanotheranostic agent[1,2]. The hydrophobic properties of IR-780 enable it to be encapsulated in the bilayer of liposome with high encapsulation efficiency. IR-780 has high singlet oxygen (1O2) quantum yield, photostability and fluorescence intensity [3], For IR-780, the 1O2 quantum yield could reach 0.127[4].
IR-780(0-160 µM;24h) induced dose-dependent inhibition of PC-3, LNCaP, and RWPE-1 cells proliferation [5].IR-780 can be efficiently taken up by RCC cells through the organic anion-transporting polypeptide (OATP) superfamily transporters, which enables the diagnosis of even small lesions through tumor tissue-specific imaging [6]. Uptake of IR-780(4 µM, 10 µM or 16 µM ;1-4h) is dose and time-dependent in 4T1 cancer cells. When these cancer cells were treated with IR-780 and US irradiation, survival was significantly reduced and necrotic/apoptotic cells increased [7].
IR-780(0.334 mg/kg and 3.334 mg/kg; i.p.; daily for 1 month) (10 times imaging dose) staining for 1 month had no significant effect on physical activity weight and histology of BABL/C mice. High signal-to-background ratios of xenografts were achieved in athymic nude mice models (IR-780 dye application in prostate cancer detection) 24 h after NIRF dyes administration [6]. Using intracranial glioma xenograft in nude mice and administration of ILs (including IR-780) by convection enhanced delivery (CED) to overcome blood-brain barrier, liposomal IR-780(0.8 g/L IR-780) could be specifically delivered to the brain tumor [8].
References:
[1]. Li S, Zhou S, et,al. Exceptionally High Payload of the IR780 Iodide on Folic Acid-Functionalized Graphene Quantum Dots for Targeted Photothermal Therapy. ACS Appl Mater Interfaces. 2017 Jul 12;9(27):22332-22341. doi: 10.1021/acsami.7b07267. Epub 2017 Jun 28. Erratum in: ACS Appl Mater Interfaces. 2020 Jun 17;12(24):27819-27820. PMID: 28643511.
[2]. Wang K, Zhang Y, et,al. Self-assembled IR780-loaded transferrin nanoparticles as an imaging, targeting and PDT/PTT agent for cancer therapy. Sci Rep. 2016 Jun 6;6:27421. doi: 10.1038/srep27421. PMID: 27263444; PMCID: PMC4899881.
[3]. Zhang L, Wang D, Yang K, Sheng D, Tan B, Wang Z, Ran H, Yi H, Zhong Y, Lin H, Chen Y. Mitochondria-Targeted Artificial "Nano-RBCs" for Amplified Synergistic Cancer Phototherapy by a Single NIR Irradiation. Adv Sci (Weinh). 2018 May 21;5(8):1800049. doi: 10.1002/advs.201800049. PMID: 30128231; PMCID: PMC6097143.
[4]. Ren H, Liu J, et,al. Relighting Photosensitizers by Synergistic Integration of Albumin and Perfluorocarbon for Enhanced Photodynamic Therapy. ACS Appl Mater Interfaces. 2017 Feb 1;9(4):3463-3473. doi: 10.1021/acsami.6b14885. Epub 2017 Jan 18. PMID: 28067039.
[5]. Yi X, Yan F, et,al. IR-780 dye for near-infrared fluorescence imaging in prostate cancer. Med Sci Monit. 2015 Feb 16;21:511-7. doi: 10.12659/MSM.892437. PMID: 25686161; PMCID: PMC4335586.
[6]. Yang X, Shao C, et,al. Optical imaging of kidney cancer with novel near infrared heptamethine carbocyanine fluorescent dyes. J Urol. 2013 Feb;189(2):702-710. doi: 10.1016/j.juro.2012.09.056. Epub 2012 Sep 20. PMID: 23000848; PMCID: PMC4120709.
[7]. Li Y, Zhou Q, et,al. IR-780 Dye as a Sonosensitizer for Sonodynamic Therapy of Breast Tumor. Sci Rep. 2016 May 13;6:25968. doi: 10.1038/srep25968. PMID: 27174006; PMCID: PMC4865802.
[8]. Lu YJ, S AT, et,al. Liposomal IR-780 as a Highly Stable Nanotheranostic Agent for Improved Photothermal/Photodynamic Therapy of Brain Tumors by Convection-Enhanced Delivery. Cancers (Basel). 2021 Jul 22;13(15):3690. doi: 10.3390/cancers13153690. PMID: 34359590; PMCID: PMC8345063.
IR 780 是一种七次甲基花青荧光探针,用于肿瘤细胞的体内成像,吸收光谱750-830nm。它也是一种强大而稳定的纳米热剂[1,2]。IR-780的疏水特性使其能够被包封在脂质体的双分子层中,具有较高的包封效率。IR-780具有较高的单线态氧(1O2)量子产率、光稳定性和荧光强度[3],1O2量子产率可达0.127[4]。
IR-780(0-160 µM;24h)对PC-3、LNCaP和RWPE-1细胞增殖有剂量依赖性抑制作用。IR-780可通过有机阴离子转运多肽(OATP)超家族转运蛋白被RCC细胞有效吸收,通过肿瘤组织特异性成像诊断小病变[6]。IR-780(4 µM、10 µM或16 µM;1-4h)在4T1癌细胞中的摄取呈剂量和时间依赖性。当这些癌细胞接受IR-780碘化物和US照射时,存活率显著降低,坏死/凋亡细胞增加[7]。
IR-780(0.334 mg/kg和3.334 mg/kg;i.p)(10倍成像剂量)染色1个月对BABL/C小鼠的运动、体重和组织学无显著影响[6]。采用裸鼠异种脑胶质瘤移植,通过ILs(含有0.8 g/L IR-780的复合物) 给药可以克服血脑屏障,特异性地向脑肿瘤递送IR-780[8]。
| Cell experiment [1]: | |
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Cell lines |
PC-3, LNCaP, and RWPE-1 cells |
|
Preparation Method |
Cells were seeded in 96-well plates (5x103 cells/well) and continued to grow 12 h for cell adhesion. After 24-h incubation with IR-780, cells were assessed for the inhibition of cell proliferation after 24 h exposure using the CCK-8 assay kit. |
|
Reaction Conditions |
0-160µM;24h |
|
Applications |
IR-780 induced dose-dependent inhibition of cell proliferation. |
| Animal experiment [2]: | |
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Animal models |
BALB/C mice |
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Preparation Method |
PC-3 cells were implanted subcutaneously into 3 athymic nude mice, besides, 3 athymic nude mice without inoculation as normal controls. When tumor sizes reached about 5-10 mm in diameter. IR-780 dye was injected i.p. at a dose of 0.334 mg/kg. The mice were anesthetized 24 h after IR-780 dye injection by 2% isoflurane in 100% oxygen with a delivery rate of 1.5 L/min. The whole-body investigate near-infrared fluorescence (NIRF) imaging of normal and tumor-loaded mice was taken using a IVIS Lumina II imaging station. |
|
Dosage form |
0.334 mg/kg; i.p. |
|
Applications |
High signal-to-background ratios of xenografts were achieved in athymic nude mice models(IR-780 dye application in prostate cancer detection)24 h after NIRF dyes administration. |
|
References: [1]. Yi X, Yan F, et,al . IR-780 dye for near-infrared fluorescence imaging in prostate cancer. Med Sci Monit. 2015 Feb 16;21:511-7. doi: 10.12659/MSM.892437. PMID: 25686161; PMCID: PMC4335586. |
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| Cas No. | 207399-07-3 | SDF | |
| 别名 | IR-780碘化物 | ||
| Canonical SMILES | ClC1=C(/C=C/C2=[N+](CCC)C3=C(C2(C)C)C=CC=C3)CCC/C1=C\C=C4N(CCC)C5=C(C/4(C)C)C=CC=C5.[I-] | ||
| 分子式 | C36H44ClN2•I | 分子量 | 667.1 |
| 溶解度 | Chloroform: 10 mg/ml,DMF: slightly soluble,DMSO: slightly soluble,Ethanol: slightly soluble | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.499 mL | 7.4951 mL | 14.9903 mL |
| 5 mM | 0.2998 mL | 1.499 mL | 2.9981 mL |
| 10 mM | 0.1499 mL | 0.7495 mL | 1.499 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet