IR 780
(Synonyms: IR-780碘化物) 目录号 : GC48396
IR 780 是一种七次甲基花青荧光探针,用于肿瘤细胞的体内成像,吸收光谱750-830nm。
Cas No.:207399-07-3
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
| Cell experiment [1]: | |
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Cell lines |
PC-3, LNCaP, and RWPE-1 cells |
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Preparation Method |
Cells were seeded in 96-well plates (5x103 cells/well) and continued to grow 12 h for cell adhesion. After 24-h incubation with IR-780, cells were assessed for the inhibition of cell proliferation after 24 h exposure using the CCK-8 assay kit. |
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Reaction Conditions |
0-160µM;24h |
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Applications |
IR-780 induced dose-dependent inhibition of cell proliferation. |
| Animal experiment [2]: | |
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Animal models |
BALB/C mice |
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Preparation Method |
PC-3 cells were implanted subcutaneously into 3 athymic nude mice, besides, 3 athymic nude mice without inoculation as normal controls. When tumor sizes reached about 5-10 mm in diameter. IR-780 dye was injected i.p. at a dose of 0.334 mg/kg. The mice were anesthetized 24 h after IR-780 dye injection by 2% isoflurane in 100% oxygen with a delivery rate of 1.5 L/min. The whole-body investigate near-infrared fluorescence (NIRF) imaging of normal and tumor-loaded mice was taken using a IVIS Lumina II imaging station. |
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Dosage form |
0.334 mg/kg; i.p. |
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Applications |
High signal-to-background ratios of xenografts were achieved in athymic nude mice models(IR-780 dye application in prostate cancer detection)24 h after NIRF dyes administration. |
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References: [1]. Yi X, Yan F, et,al . IR-780 dye for near-infrared fluorescence imaging in prostate cancer. Med Sci Monit. 2015 Feb 16;21:511-7. doi: 10.12659/MSM.892437. PMID: 25686161; PMCID: PMC4335586. |
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IR 780 is a heptacyanine fluorescent probe for in vivo imaging of tumor cells with a large absorption spectrum of 750-830nm.It is also a powerful and stable nanotheranostic agent[1,2]. The hydrophobic properties of IR-780 enable it to be encapsulated in the bilayer of liposome with high encapsulation efficiency. IR-780 has high singlet oxygen (1O2) quantum yield, photostability and fluorescence intensity [3], For IR-780, the 1O2 quantum yield could reach 0.127[4].
IR-780(0-160 µM;24h) induced dose-dependent inhibition of PC-3, LNCaP, and RWPE-1 cells proliferation [5].IR-780 can be efficiently taken up by RCC cells through the organic anion-transporting polypeptide (OATP) superfamily transporters, which enables the diagnosis of even small lesions through tumor tissue-specific imaging [6]. Uptake of IR-780(4 µM, 10 µM or 16 µM ;1-4h) is dose and time-dependent in 4T1 cancer cells. When these cancer cells were treated with IR-780 and US irradiation, survival was significantly reduced and necrotic/apoptotic cells increased [7].
IR-780(0.334 mg/kg and 3.334 mg/kg; i.p.; daily for 1 month) (10 times imaging dose) staining for 1 month had no significant effect on physical activity weight and histology of BABL/C mice. High signal-to-background ratios of xenografts were achieved in athymic nude mice models (IR-780 dye application in prostate cancer detection) 24 h after NIRF dyes administration [6]. Using intracranial glioma xenograft in nude mice and administration of ILs (including IR-780) by convection enhanced delivery (CED) to overcome blood-brain barrier, liposomal IR-780(0.8 g/L IR-780) could be specifically delivered to the brain tumor [8].
References:
[1]. Li S, Zhou S, et,al. Exceptionally High Payload of the IR780 Iodide on Folic Acid-Functionalized Graphene Quantum Dots for Targeted Photothermal Therapy. ACS Appl Mater Interfaces. 2017 Jul 12;9(27):22332-22341. doi: 10.1021/acsami.7b07267. Epub 2017 Jun 28. Erratum in: ACS Appl Mater Interfaces. 2020 Jun 17;12(24):27819-27820. PMID: 28643511.
[2]. Wang K, Zhang Y, et,al. Self-assembled IR780-loaded transferrin nanoparticles as an imaging, targeting and PDT/PTT agent for cancer therapy. Sci Rep. 2016 Jun 6;6:27421. doi: 10.1038/srep27421. PMID: 27263444; PMCID: PMC4899881.
[3]. Zhang L, Wang D, Yang K, Sheng D, Tan B, Wang Z, Ran H, Yi H, Zhong Y, Lin H, Chen Y. Mitochondria-Targeted Artificial "Nano-RBCs" for Amplified Synergistic Cancer Phototherapy by a Single NIR Irradiation. Adv Sci (Weinh). 2018 May 21;5(8):1800049. doi: 10.1002/advs.201800049. PMID: 30128231; PMCID: PMC6097143.
[4]. Ren H, Liu J, et,al. Relighting Photosensitizers by Synergistic Integration of Albumin and Perfluorocarbon for Enhanced Photodynamic Therapy. ACS Appl Mater Interfaces. 2017 Feb 1;9(4):3463-3473. doi: 10.1021/acsami.6b14885. Epub 2017 Jan 18. PMID: 28067039.
[5]. Yi X, Yan F, et,al. IR-780 dye for near-infrared fluorescence imaging in prostate cancer. Med Sci Monit. 2015 Feb 16;21:511-7. doi: 10.12659/MSM.892437. PMID: 25686161; PMCID: PMC4335586.
[6]. Yang X, Shao C, et,al. Optical imaging of kidney cancer with novel near infrared heptamethine carbocyanine fluorescent dyes. J Urol. 2013 Feb;189(2):702-710. doi: 10.1016/j.juro.2012.09.056. Epub 2012 Sep 20. PMID: 23000848; PMCID: PMC4120709.
[7]. Li Y, Zhou Q, et,al. IR-780 Dye as a Sonosensitizer for Sonodynamic Therapy of Breast Tumor. Sci Rep. 2016 May 13;6:25968. doi: 10.1038/srep25968. PMID: 27174006; PMCID: PMC4865802.
[8]. Lu YJ, S AT, et,al. Liposomal IR-780 as a Highly Stable Nanotheranostic Agent for Improved Photothermal/Photodynamic Therapy of Brain Tumors by Convection-Enhanced Delivery. Cancers (Basel). 2021 Jul 22;13(15):3690. doi: 10.3390/cancers13153690. PMID: 34359590; PMCID: PMC8345063.
IR 780 是一种七次甲基花青荧光探针,用于肿瘤细胞的体内成像,吸收光谱750-830nm。它也是一种强大而稳定的纳米热剂[1,2]。IR-780的疏水特性使其能够被包封在脂质体的双分子层中,具有较高的包封效率。IR-780具有较高的单线态氧(1O2)量子产率、光稳定性和荧光强度[3],1O2量子产率可达0.127[4]。
IR-780(0-160 µM;24h)对PC-3、LNCaP和RWPE-1细胞增殖有剂量依赖性抑制作用。IR-780可通过有机阴离子转运多肽(OATP)超家族转运蛋白被RCC细胞有效吸收,通过肿瘤组织特异性成像诊断小病变[6]。IR-780(4 µM、10 µM或16 µM;1-4h)在4T1癌细胞中的摄取呈剂量和时间依赖性。当这些癌细胞接受IR-780碘化物和US照射时,存活率显著降低,坏死/凋亡细胞增加[7]。
IR-780(0.334 mg/kg和3.334 mg/kg;i.p)(10倍成像剂量)染色1个月对BABL/C小鼠的运动、体重和组织学无显著影响[6]。采用裸鼠异种脑胶质瘤移植,通过ILs(含有0.8 g/L IR-780的复合物) 给药可以克服血脑屏障,特异性地向脑肿瘤递送IR-780[8]。
| Cas No. | 207399-07-3 | SDF | |
| 别名 | IR-780碘化物 | ||
| Canonical SMILES | ClC1=C(/C=C/C2=[N+](CCC)C3=C(C2(C)C)C=CC=C3)CCC/C1=C\C=C4N(CCC)C5=C(C/4(C)C)C=CC=C5.[I-] | ||
| 分子式 | C36H44ClN2•I | 分子量 | 667.1 |
| 溶解度 | Chloroform: 10 mg/ml,DMF: slightly soluble,DMSO: slightly soluble,Ethanol: slightly soluble | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.499 mL | 7.4951 mL | 14.9903 mL |
| 5 mM | 0.2998 mL | 1.499 mL | 2.9981 mL |
| 10 mM | 0.1499 mL | 0.7495 mL | 1.499 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
IR 780-loaded hyaluronic acid micelles for enhanced tumor-targeted photothermal therapy
Carbohydr Polym 2018 Feb 1;181:1-9.PMID:29253923DOI:10.1016/j.carbpol.2017.10.033.
In this study, we propose using IR 780-loaded, CD44-targeted hyaluronic acid-based micelles (HA-IR 780) for enhanced photothermal therapy (PTT) effects in tumors. Two kinds of HA-C18 micelles were synthesized from different C18 feed ratios with degree of substitution of 3% and 13% respectively. Three different IR 780 weight percentages were used for micelle formation with loading content of 4.6%, 7.9%, and 10.3% respectively. The IC50 value of HA-IR 780 in TC1 cells was 21.89μgmL-1 (32.81μM). Upon irradiation of the tumor site with an 808-nm laser (2Wcm-2) for 2min, the temperature in the tumor in the HA-IR 780-treated groups reached 49.9°C which exceeds the temperature threshold to induce irreversible tissue damage. Toxicity studies showed that HA-IR 780 does not cause any adverse effects in organs, including heart, liver, lungs, kidney and spleen, although it selectively caused cell damage in the tumor region upon laser irradiation. Therefore, the present study suggests that HA-IR 780 can cause selective cell death in tumor regions due to its enhanced tumor-targeting and photothermal capabilities.
An Opto- and Thermal-Rewrite PCM/CNF-IR 780 Energy Storage Nanopaper with Mechanical Regulated Performance
Small 2022 Jun;18(25):e2200688.PMID:35599429DOI:10.1002/smll.202200688.
In spite of efforts to fabricate self-assembled energy storage nanopaper with potential applications in displays, greenhouses, and sensors, few studies have investigated their multiple stimuli-sensitivities. Here, an opto- and thermal-rewrite phase change material/cellulose nanofibril (PCM/CNF) energy storage nanopaper with mechanical regulated performance is facilely fabricated, through 5 min sonication of PCMs and CNFs in an aqueous system. The combination of PCM and CNF not only guarantees the recyclability of PCM without leakage, but also offers nanopaper adaptive properties by leveraging the mobility and optical variation accompanying solid-to-liquid transition of PCM. Besides, trace near-infrared (NIR) dye (IR 780) in it imparts a PCM-embedded nanopaper photothermal effect to modulate the local transparency via time- and position-controlled laser exposure, leading to a reusable opto-writing nanopaper. Furthermore, since the synergistic effect of stick-and-slip function attributes from PCMs and pore structures are produced by calcium ions, the PCM/CNF energy storage nanopaper exhibits excellent mechanically regulated performance from rigid to flexible, which greatly enriches their application in energy-efficient smart buildings and displays.