Isatin
(Synonyms: 靛红,Indoline-2,3-dione) 目录号 : GC12962Isatin (Indoline-2,3-dione) 是一种有效的单胺氧化酶 (MAO) 抑制剂,IC50 为 3 μM。
Cas No.:91-56-5
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
IC50: 15 μM
Isatin is a monoamine oxidase inhibitor.
Monoamine oxidase inhibitors have been used as therapies for the treatment of depression, particularly in treating atypical depression. Monoamine oxidase inhibitors are also used in the treatment of Parkinson's disease and other disorders.
In vitro: Previous study found that isatin treatment at 1-400 μM for 24h could induce a significant dose-dependent increase in MTT metabolism by SH-SY5Y cells, which was not due to the increase in cell division. In addition, isatin at the higher concentrations was able to trigger cell death, though MTT metabolism was still increased, indicating that the surviving cells were hypermetabolic. With a longer treatment, isatin was found to cause cell death in a dose-dependent manner, and the predominant mode of cell death was apoptosis at lower concentrations, while at the highest concentration increasing numbers of necrotic cells were also observed [1].
In vivo: Animal study showed that the motor activity of Japanese encephalitis virus (JEV)-induced rats receiving isatin was improved significantly when compared with that of untreated JEV-infected rats. In addition, isatin was able to prevent the decrease in striatal DA levels in JEV-rats and the increased turnover of dopamine (DA) (DOPAC/DA) induced by JEV was significantly inhibited by isatin. Such results indicated that the exogenously administered isatin was able to improve JEV-induced parkinsonism via increasing the striatum DA concentrations [2].
Clinical trial: Up to now, isatin is still in the preclinical development stage.
References:
[1] Igosheva N,Lorz C,O'Conner E,Glover V,Mehmet H. Isatin, an endogenous monoamine oxidase inhibitor, triggers a dose- and time-dependent switch from apoptosis to necrosis in human neuroblastoma cells. Neurochem Int.2005 Aug;47(3):216-24.
[2] Hamaue N,Minami M,Terado M,Hirafuji M,Endo T,Machida M,Hiroshige T,Ogata A,Tashiro K,Saito H,Parvez SH. Comparative study of the effects of isatin, an endogenous MAO-inhibitor, and selegiline on bradykinesia and dopamine levels in a rat model of Parkinson's disease induced by the Japanese encephalitis virus. Neurotoxicology.2004 Jan;25(1-2):205-13.
Cas No. | 91-56-5 | SDF | |
别名 | 靛红,Indoline-2,3-dione | ||
化学名 | indoline-2,3-dione | ||
Canonical SMILES | O=C(C1=O)C2=C(N1)C=CC=C2 | ||
分子式 | C8H5NO2 | 分子量 | 147.13 |
溶解度 | DMSO : 29mg/mL | 储存条件 | Store at -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
||
Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 6.7967 mL | 33.9836 mL | 67.9671 mL |
5 mM | 1.3593 mL | 6.7967 mL | 13.5934 mL |
10 mM | 0.6797 mL | 3.3984 mL | 6.7967 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。