Isodeoxycholic Acid
(Synonyms: isoDCA) 目录号 : GC47464
A bile acid
Cas No.:566-17-6
Sample solution is provided at 25 µL, 10mM.
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Isodeoxycholic acid is a bile acid that is formed via epimerization of deoxycholic acid by intestinal bacteria.[1] It has a greater critical micelle concentration than DCA, indicating reduced detergent activity, and is less active than DCA in inhibiting growth in a panel of seven gut commensal bacteria species. Isodeoxycholic acid (0.1%) inhibits spore germination induced by taurocholic acid in several C. difficile strains, as well as decreases the cytotoxicity of C. difficile culture supernatants to Vero cells.[2] Plasma levels of isodeoxycholic acid are decreased in a rat model of high-fat diet-induced obesity compared with rats fed a normal diet.[3]
Reference:
[1].Devlin, A.S., and Fischbach, M.A.A biosynthetic pathway for a prominent class of microbiota-derived bile acidsNat. Chem. Biol.11(9)685-690(2018)
[2].Thanissery, R., Winston, J.A., and Theriot, C.M.Inhibition of spore germination, growth, and toxin activity of clinically relevant C. difficile strains by gut microbiota derived secondary bile acidsAnaerobe4586-100(2017)
[3].Lin, H., An, Y., Tang, H., et al.Alterations of bile acids and gut microbiota in obesity induced by high fat diet in rat modelJ. Agric. Food Chem.67(13)3624-3632(2019)
| Cas No. | 566-17-6 | SDF | |
| 别名 | isoDCA | ||
| 化学名 | (5β,7α,12α)-7,12-dihydroxy-cholan-24-oic acid | ||
| Canonical SMILES | C[C@@]12[C@@](C[C@@H](O)[C@]3([H])[C@]2([H])C[C@H](O)[C@@]4(C)[C@@]3([H])CC[C@]4([H])[C@H](C)CCC(O)=O)([H])CCCC1 | ||
| 分子式 | C24H40O4 | 分子量 | 392.6 |
| 溶解度 | DMF: 30mg/mL,DMF:PBS (pH 7.2) (1:1): 0.5mg/mL,DMSO: 20mg/mL,Ethanol: 20mg/mL | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.5471 mL | 12.7356 mL | 25.4712 mL |
| 5 mM | 0.5094 mL | 2.5471 mL | 5.0942 mL |
| 10 mM | 0.2547 mL | 1.2736 mL | 2.5471 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet