Isoginkgetin
(Synonyms: 异银杏素) 目录号 : GC32758
A biflavonoid inhibitor of pre-mRNA splicing
Cas No.:548-19-6
Sample solution is provided at 25 µL, 10mM.
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Isoginkgetin is a biflavonoid that has been found in G. biloba and is an inhibitor of pre-mRNA splicing.1 Isoginkgetin (33 μM) reduces the activity of an mRNA-dependent luciferase reporter by 5-fold, shifts the composition of total RNA extract from predominantly mRNA to pre-mRNA, and arrests cell growth in HEK293 cells. Isoginkgetin (10 μM) suppresses mouse lymphocyte proliferation induced by concanavalin A and LPS by approximately 87 and 90%, respectively.2 It also suppresses arachidonic acid release induced by phorbol 12-myristate 13-acetate and the calcium ionophore A23187 by 32.5 and 48.4%, respectively, in rat peritoneal macrophages when used at a concentration of 10 μM.3 Isoginkgetin (5-20 μM) reduces matrix metalloproteinase-9 (MMP-9) activity, expression, and mRNA levels in and decreases cell invasion by HT1080 fibrosarcoma cells in a concentration-dependent manner.4
1.O'Brien, K., Matlin, A.J., Lowell, A.M., et al.The biflavonoid isoginkgetin is a general inhibitor of pre-mRNA splicingJ. Biol. Chem.283(48)33147-33154(2008) 2.Lee, S.J., Choi, J.H., Son, K.H., et al.Suppression of mouse lymphocyte proliferation in vitro by naturally-occurring biflavonoidsLife Sci.57(6)551-558(1995) 3.Lee, S.J., Son, K.H., Chang, H.W., et al.Inhibition of arachidonate release from rat peritoneal macrophage by biflavonoidsArch. Pharm. Res.20(6)533-538(1997) 4.Yoon, S.-O., Shin, S.S., Lee, H.-J., et al.Isoginkgetin inhibits tumor cell invasion by regulating phosphatidylinositol 3-kinase/Akt-dependent matrix metalloproteinase-9 expressionMol. Cancer Ther.5(11)2666-2675(2006)
| Cas No. | 548-19-6 | SDF | |
| 别名 | 异银杏素 | ||
| Canonical SMILES | O=C1C=C(C2=CC=C(OC)C=C2)OC3=C(C4=CC(C5=CC(C6=C(O)C=C(O)C=C6O5)=O)=CC=C4OC)C(O)=CC(O)=C13 | ||
| 分子式 | C32H22O10 | 分子量 | 566.51 |
| 溶解度 | DMSO : ≥ 83.3 mg/mL (147.04 mM) | 储存条件 | Store at 2-8°C,protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.7652 mL | 8.826 mL | 17.6519 mL |
| 5 mM | 0.353 mL | 1.7652 mL | 3.5304 mL |
| 10 mM | 0.1765 mL | 0.8826 mL | 1.7652 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet