Isoprothiolane
(Synonyms: 稻瘟灵) 目录号 : GC63025
Isoprothiolane 是一种全身性杀菌剂 (fungicide)。Isoprothiolane 是稻瘟病 (fungal disease) 防治剂，对 Pyvioutavia oryzae Cav 导致的虫病有防治作用。
Cas No.:50512-35-1
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Isoprothiolane is a systemic fungicide. Isoprothiolane is a rice blast controlling agent against the fungal disease of rice planty Pyvioutavia oryzae Cav[1].
[1]. Matazaemon Uchida, et al. Effect of a Rice Blast Controlling Agent, Isoprothiolane, on Nilaparvata Lugens Stal with Different Levels of Susceptibility to Diazinon. Pest Resistance to Pesticides pp 421-428
| Cas No. | 50512-35-1 | SDF | |
| 别名 | 稻瘟灵 | ||
| 分子式 | C12H18O4S2 | 分子量 | 290.4 |
| 溶解度 | DMSO : 100 mg/mL (344.35 mM; Need ultrasonic) | 储存条件 | |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.4435 mL | 17.2176 mL | 34.4353 mL |
| 5 mM | 0.6887 mL | 3.4435 mL | 6.8871 mL |
| 10 mM | 0.3444 mL | 1.7218 mL | 3.4435 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Oxidation of Isoprothiolane by ozone and chlorine: Reaction kinetics and mechanism
Chemosphere 2019 Oct;232:516-525.PMID:31160048DOI:10.1016/j.chemosphere.2019.03.179.
Isoprothiolane (IPT) was one of the most commonly used pesticides around the world. It was reported to be the highest concentration and frequency of detection of 13 most commonly used pesticides in Mekong Delta recently. The oxidation degradation kinetic of ozone and chlorine with IPT and the identification of the degradation products was investigated in this research. The results showed that both ozone and chlorine oxidized IPT rapidly under typical water treatment condition, and that both reactions followed second order reaction kinetics. The ozone reaction rates exhibited no pH dependence with the rate constant of 247.1 (±11.0) M-1s-1 at 25 °C, whereas chlorine reaction rates increased dramatically with decreasing pH. The rate constant for hypochloric acid was 73.3 (±3.1) M-1s-1 at 25 °C, while the reaction of hypochlorite was negligible. The degradation products by chlorine and ozone were identified by LC-MS/MS and the reaction pathways were proposed. The thioether and the carbon-carbon double bond in IPT were the reactive sites during chlorine and ozone oxidation. The thioether group was oxidized into sulfoxide and further sulfone group, and the carbon-carbon double bone were cleaved to form diisopropyl ester of malonic acid, diisopropyl ester of tartronic acid and diisopropyl ester of ketomalonic acid monohydrate. Compared to ozone reaction, it was more complicated for chlorine reaction, which yielded chlorine substituted, hydroxylated and dithiolane ring-opening products.
Behavior of Isoprothiolane and fipronil in paddy water, soil, and rice plants after nursery-box or submerged applications
J Pestic Sci 2018 May 20;43(2):132-141.PMID:30363141DOI:10.1584/jpestics.D17-083.
We investigated the behavior of Isoprothiolane and fipronil after nursery-box application and that of Isoprothiolane after submerged application in an experimental paddy field. The concentrations of the pesticides and their metabolites were monitored in paddy water, soil, and rice plants. The distribution profile for Isoprothiolane mass in the field differed greatly between the nursery-box and submerged applications. The nursery-box-applied pesticides were mostly distributed in soil near the transplanted rice seedlings (root zone), versus little distribution in paddy water and rice plants (<1.1 and <0.3% of the applied mass, respectively). The residual levels in rice plants were similar to those in the root-zone soil. To estimate the soil pesticide mass, we defined a key parameter: the ratio of the root-zone area to the total area of the paddy field estimated to be 0.1 to 0.15. This parameter is important when evaluating the concentrations of nursery-box-applied pesticides in soil and rice plants.
Detection of Isoprothiolane in food, soil, and water samples by immunosorbent assay using avian antibodies
J Immunoassay Immunochem 2013;34(2):149-65.PMID:23537300DOI:10.1080/15321819.2012.699492.
A simple competitive immunoassay was developed for the measurement of Isoprothiolane in rice, soil, and water samples. It employed the avian antibodies (IgY) that recognized Isoprothiolane as a capture reagent and isoprothiolane-alkaline phosphatase conjugate as an enzyme label. The assay depended on the competitive binding between the anti-isoprothiolane antibody and Isoprothiolane derived from rice, soil, and water samples for binding sites with immobilized isoprothiolane-ovalbumin (OVA) conjugate. The concentration of Isoprothiolane in the rice, soil, and water samples was quantified by the ability of the pesticide present in the samples to inhibit the binding of the enzyme conjugate to the antibody and subsequently the color formation in the assay. The assay was specific to Isoprothiolane with a limit of detection of 2 ng/mL. Mean analytical recovery of Isoprothiolane in different rice matrices was 87.20%-98.02%, for soil samples recovery was 74.24%-111.20%, and water samples recovery was 35.2%-95.73%. The precision of the assay was satisfactory. The assay compared favorably with gas chromatography (GC) in its ability to accurately measure Isoprothiolane in the different rice, soil, and water samples.
Activation of cell proliferation in Arabidopsis root meristem by Isoprothiolane
J Pestic Sci 2018 Nov 20;43(4):261-265.PMID:30479547DOI:10.1584/jpestics.D18-043.
A plant growth regulating agent "Fuji-one" has been used to control non-parasitic damping-off (Murenae disease) of rice seedlings. Its active ingredient, Isoprothiolane (diisopropyl 1,3-dithiolan-2-ylidenemalonate, IPT), enhances root elongation of rice and Arabidopsis. To understand the mechanisms of IPT's effect on root development, its effect on Arabidopsis root cells was investigated histologically. IPT at a lower concentration (12.5 µg/mL) had no effect on root cell elongation, whereas it enhanced cell division in the root meristem. Histological analysis using phytohormone-related mutants indicated that jasmonic acid and ethylene were involved in the enhanced cell division. In contrast, IPT at a higher concentration (75 µg/mL) suppressed both cell elongation and cell division, in which jasmonic acid and ethylene were not involved. In addition, root hair formation was suppressed by treatment with IPT. These analyses demonstrated that IPT (12.5 µg/mL) enhanced root elongation by activating cell division in a jasmonic acid- and ethylene-dependent manner.
Involvement of phytohormones in root elongation activity of Isoprothiolane in Arabidopsis
J Pestic Sci 2018 Aug 20;43(3):186-190.PMID:30363117DOI:10.1584/jpestics.D18-019.
Isoprothiolane (diisopropyl 1,3-dithiolan-2-ylidenemalonate, IPT), an active ingredient of "Fuji-one," has been used as a plant growth regulating agent to control non-parasitic damping-off (MURENAE disease) of rice seedlings. To understant plant growth regulating activity of IPT, its effect on root development was investigated in Arabidopsis. IPT enhanced root elongation at a lower concentration (12.5 µg/mL) but suppressed it at a higher concentration (75 µg/mL). Analysis using phytohormone-related mutants and chemical inhibitors revealed that the enhancement of root elongation by IPT required auxin, jasmonic acid, and ethylene signal transduction. Activation of the signal transduction mediated by these three phytohormones was confirmed by gene expression analysis. More detailed mechanisms of IPT's effect on root development were demonstrated via investigation using Arabidopsis and chemical inhibitors.