Isosulfan blue
(Synonyms: 提纯专利兰紫) 目录号 : GC39176
Isosulfan Blue (Lymphazurin dye) is a blue dye used as a visual imaging agent in lymph node mapping procedures.
Cas No.:68238-36-8
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Isosulfan Blue (Lymphazurin dye) is a blue dye used as a visual imaging agent in lymph node mapping procedures.
[1] Vinay Goswamy, et al. Journal of Clinical Oncology Volume 36, Issue 15
| Cas No. | 68238-36-8 | SDF | |
| 别名 | 提纯专利兰紫 | ||
| Canonical SMILES | CC/[N+](CC)=C1C=C/C(C=C/1)=C(C2=CC=C(N(CC)CC)C=C2)\C3=CC(S(=O)([O-])=O)=CC=C3S(=O)([O-])=O.[Na+] | ||
| 分子式 | C27H31N2NaO6S2 | 分子量 | 566.66 |
| 溶解度 | DMSO: 83.33 mg/mL (147.05 mM) | 储存条件 | Store at -20°C, protect from light, filled inert atmosphere |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.7647 mL | 8.8236 mL | 17.6473 mL |
| 5 mM | 0.3529 mL | 1.7647 mL | 3.5295 mL |
| 10 mM | 0.1765 mL | 0.8824 mL | 1.7647 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Isosulfan blue and Anaphylaxis
Ochsner J 2021 Winter;21(4):419-424.PMID:34984060DOI:10.31486/toj.20.0162.
Background: Isosulfan blue dye, or Lymphazurin, is commonly used for sentinel lymph node biopsy during operative procedures for patients with breast cancer. Allergic reactions to Lymphazurin have been reported, ranging from mild dermatologic reactions to severe anaphylaxis. Case Series: We report 2 patients who experienced allergic reaction to Lymphazurin while admitted to our service. We also conducted a literature search for similar cases using national databases. Included studies were limited to retrospective studies, case series, or case reports. Patient characteristics, reaction observed, and hospital course were extracted. Of the patients we report, both had grade 3 anaphylactic reactions requiring vasopressors to achieve hemodynamic stability. One patient required intensive care unit monitoring for 18 hours, and the other required overnight monitoring in the postanesthesia care unit. The literature revealed 29 studies reporting 108 patients with confirmed allergic reactions to Lymphazurin. Including the 2 patients in this series (total study n=110), most reactions were grade 3 (57/110, 51.8%), followed by grade 1 (40/110, 36.4%) and grade 2 (13/110, 11.8%). Among the patients who had individual hospital course reported (n=34), 23 patients required admission to the surgical intensive care unit. Of studies that reported cancellation or progression of surgery after the reaction, the surgical procedure was canceled for 12 of 26 patients (46.1%). Conclusion: Although severe anaphylactic reactions are more commonly reported, mild reactions occur more frequently but are likely underreported. Although no fatalities were reported in the cases included in this review, anaphylactic reactions to Lymphazurin pose significant risks. Operating room personnel should be familiar with potential reactions to recognize and treat them early.
Case Report: Isosulfan Blue-Related Anaphylaxis
Mil Med 2023 Jan 5;usac419.PMID:36610991DOI:10.1093/milmed/usac419.
Anaphylaxis is an acute multisystem syndrome typically resulting from the sudden release of mast cell and basophil-derived mediators into the circulatory system. Isosulfan blue dye is a rare, but known, mediator of anaphylaxis with an incidence between 0.16% and 2% of cases. We report two cases of anaphylaxis attributed to the use of Isosulfan blue dye in the intraoperative setting. Both of the patients we report had grade 3 anaphylactic reactions requiring vasopressors to correct significant hypotension. Both patients required overnight monitoring in the intensive care unit with the second patient also requiring continued vasopressor support.
A comparison of the adverse reactions associated with Isosulfan blue versus methylene blue dye in sentinel lymph node biopsy for breast cancer
Am J Surg 2005 Feb;189(2):236-9.PMID:15720998DOI:10.1016/j.amjsurg.2004.06.042.
Background: Sentinel lymph node biopsy (SLNB) is an established means of staging the axilla in patients with breast cancer. Recently, methylene blue dye has been shown to be an efficacious and cost-effective alternative to Isosulfan blue. With the increasing popularity of SLNB, the potential complications of Isosulfan blue use must be appreciated. Methods: A literature search for English language articles available on MEDLINE from 1985 to November 2002 using the search terms allergy, allergic reaction, anaphylactic reactions, anaphylaxis, blue dye, breast cancer, Isosulfan blue, methylene blue, and sentinel lymph node biopsy identified 24 reports. Conclusions: The use of Isosulfan blue due for SLNB is associated with a significant number of allergic reactions, some of which are life-threatening. Because methylene blue dye has been shown to be equally effective and does not pose a serious risk of serious allergic reactions, it offers an improved technique above Isosulfan blue dye for SLNB.
Anaphylaxis to Isosulfan blue and cross-reactivity to patent blue V: case report and review of the nomenclature of vital blue dyes
Ann Allergy Asthma Immunol 2006 Mar;96(3):497-500.PMID:16597088DOI:10.1016/S1081-1206(10)60921-0.
Background: Blue dyes used for lymphatic mapping in sentinel lymph node biopsy cause intraoperative anaphylactic reactions in up to 2.7% of patients. With increasing implementation of this technique, the incidence of anaphylaxis to these dyes can be expected to increase. In the literature, the chemically often unrelated and inconsistently designated dyes have been confused, adding to other inconsistencies in the nomenclature. Objective: To demonstrate the nomenclature, chemical and physiologic differences, and allergenicity of the various blue dyes used in a medical context. Methods: We describe a patient with an intraoperative grade IV anaphylactic reaction to Isosulfan blue. Immediate-type hypersensitivity was proved by positive skin test reactions and CD63 expression to Isosulfan blue and cross-reactivity to patent blue V. Results: A review of the literature clarified the exact nomenclature of the blue dyes and the possible pitfalls of confusing nomenclature in the context of structurally closely related dyes with different allergenic properties. For the detection of type I hypersensitivity, intracutaneous tests are valuable tools. An IgE-mediated mechanism has been shown recently. In most cases, sensitization exists without known previous exposure in a medical context. This may be due to the widespread use of such dyes in objects of everyday life. Preoperative antiallergic medication use does not prevent anaphylactic reactions but apparently reduces their severity. Conclusion: For better comparison and precision, the Chemical Abstracts Service number of the respective dye should always be given.
Isosulfan blue dye reactions during sentinel lymph node mapping for breast cancer
Anesth Analg 2002 Aug;95(2):385-8, table of contents.PMID:12145056DOI:10.1097/00000539-200208000-00026.
In the United States, identification of the sentinel lymph node (SLN) requires the use of (99m)Tc-labeled colloid, 1% Isosulfan blue dye, or both to trace the lymphatic drainage of a given neoplasm. We report our experience with adverse reactions to Isosulfan blue dye during SLN mapping in breast cancer. A chart review of the breast cancer SLN database was performed; it included 2392 sequential patients who underwent SLN biopsy involving Isosulfan blue dye at Memorial Sloan-Kettering Cancer Center from September 12, 1996, to August 17, 2000. Thirty-nine of 2392 patients (1.6%) had a documented allergic reaction during the mapping procedure. Most reactions (69%) produced urticaria, blue hives, a generalized rash, or pruritus. The incidence of hypotensive reactions was 0.5%. Although anaphylaxis after the injection of Isosulfan blue dye is rare, this article highlights the need to suspect anaphylaxis when hemodynamic instability occurs after the injection of this compound. Our experience indicates that bronchospasm and respiratory compromise are unusual and that most patients do not require emergent intubation and can be managed with short-term pressor support. In addition, our data indicate that patients with a sulfa allergy do not display a cross-sensitivity to Isosulfan blue dye. Implications: We report the largest single-institution review of adverse reactions to injection of Isosulfan blue dye during sentinel lymph node mapping in breast cancer. Bronchospasm and respiratory compromise are unusual, and most patients can be treated with short-term pressor support. Patients with a sulfa allergy do not display a cross-sensitivity to Isosulfan blue dye.