Isovaleryl-Coenzyme A (sodium salt)
(Synonyms: Isovaleryl-CoA) 目录号 : GC91817Isovaleryl-coenzyme A (isovaleryl-CoA) is a branched-chain acyl CoA and an intermediate in the metabolism of the essential amino acid L-leucine .
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Isovaleryl-coenzyme A (isovaleryl-CoA) is a branched-chain acyl CoA and an intermediate in the metabolism of the essential amino acid L-leucine .[1],[2] It is formed from L-leucine by the branched-chain α-keto acid dehydrogenase (BCKAD) complex and is converted to 3-methylcrotonyl-CoA by isovaleryl-CoA dehydrogenase (IVD), an enzyme involved in the inborn error of metabolism isovaleric acidemia.[3] Isovaleryl-CoA increases the oxidation rate of the branched-chain 2-oxo acids 3-methyl-2-butanoate and 4-methyl-2-oxopentanoate in isolated rat muscle mitochondria in the absence of carnitine when used at a concentration of 1 mM.[4] It also inhibits succinate-CoA ligase activity in rat liver mitochondria (IC50 = 273 µM).[5]
References:
[1].Duan, Y., Li, F., Li, Y., et al.The role of leucine and its metabolites in protein and energy metabolismAmino Acids48(1)41-51(2016).
[2].Mohsen, A.-W.A., Anderson, B.D., Volchenboum, S.L., et al.Characterization of molecular defects in isovaleryl-CoA dehydrogenase in patients with isovaleric acidemiaBiochemistry37(28)10325-10335(1998).
[3].Mack, M., Schniegler-Mattox, U., Peters, V., et al.Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolismFEBS J.273(9)2012-2022(2006).
[4].Veerkamp, J.H., van Moerkerk, H.T.B., and Wagenmakers, A.J.M.Interaction of short-chain and branched-chain fatty acids and their carnitine and CoA esters and of various metabolites and agents with branced-chain 2-oxo acid oxidation in rat muscle and liver mitochondriaInt. J. Biochem.17(9)967-974(1985).
[5].Bergen, B.J., Stumpf, D.A., Haas, R., et al.A mechanism of toxicity of isovaleric acid in rat liver mitochondriaBiochem. Med.27(2)154-160(1982).
| Cas No. | N/A | SDF | Download SDF |
| 别名 | Isovaleryl-CoA | ||
| 分子式 | C26H44N7O17P3S•XNa | 分子量 | 851.7 |
| 溶解度 | Water: Soluble | 储存条件 | -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.1741 mL | 5.8706 mL | 11.7412 mL |
| 5 mM | 0.2348 mL | 1.1741 mL | 2.3482 mL |
| 10 mM | 0.1174 mL | 0.5871 mL | 1.1741 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。