Janelia Fluor 526, NHS ester
目录号 : GC59473Fluorogenic yellow dye (Reactive group: NHS ester). Application: confocal microscopy, two-color lattice light-sheet microscopy, super resolution microscopy
Sample solution is provided at 25 µL, 10mM.
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Key Information: Janelia Fluor® 526, NHS ester is a fluorogenic yellow fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines. Application: Suitable for confocal microscopy, two-color lattice light-sheet microscopy, super resolution microscopy (SRM) including dSTORM (in both live and fixed cells) and STED. Cell permeable. Properties and Photophysical Data: NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Can be multiplexed for two color imaging with Janelia Fluor® 635 NHS ester. Excitation and emission maxima (λ) are 526 nm and 550 nm, respectively; quantum yield = 0.87; extinction coefficient = 118,000 M-1cm-1; lactone-zwitterion equilibrium constant (KL-Z) = 0.005. To measure the absorbance spectrum of this dye we recommend the following solvent: TFE plus 0.1% TFA. Please see the protocol for further information on protein/antibody labeling and conjugation. We also offer Janelia Fluor® conjugated antibodies and custom conjugation services. HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc. Janelia Fluor is a registered trademark of Howard Hughes Medical Institute. This product has been recently renamed. The previous name for this product was Janelia Fluor® 526, SE.
| Cas No. | SDF | ||
| 化学名 | 2-(3-(3,3-Difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-2,7-difluoro-3H-xanthen-9-yl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)benzoate | ||
| Canonical SMILES | O=C(ON1C(CCC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC(F)=C(N4CC(F)(F)C4)C=C3O5)=C(C=C/6F)C5=CC6=[N+]7CC(F)(F)C\7)=C2 | ||
| 分子式 | C31H19F6N3O7 | 分子量 | 659.5 |
| 溶解度 | DMSO: 13.19mg/mL | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.5163 mL | 7.5815 mL | 15.163 mL |
| 5 mM | 0.3033 mL | 1.5163 mL | 3.0326 mL |
| 10 mM | 0.1516 mL | 0.7582 mL | 1.5163 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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