Kanamycin A
(Synonyms: 卡那霉素; 卡那霉素 A; Kanamycin A) 目录号 : GC16515
An antibiotic used in molecular biology
Cas No.:59-01-8
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
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Kanamycin A is a broad spectrum antibiotic.
Kanamycin A was first isolated in 1957 by Hamao Umezawa from the bacterium Streptomyces kanamyceticus.
In vitro: In previous study, kanamycin A was found to block a single cycle of translocation on the poly[U]-ribosome, carrying N-acetyl-diPhe-tRNA on the acceptor site and deacylated tRNA at the donor site. The GTPase reaction, catalyzed by EF-G and ribosomes, was not significantly affected by kanamycin A. The results with kanamycin A differed from those obtained with fusidic acid, suggesting that the mechanism of translocation inhibition might be differen [1].
In vivo: Twelve male Lister hooded rats were conditioned to discriminate an 8 kHz tone and were subsequently injected subcutaneously with kanamycin A for 28 days. Results showed that only one rat was unaffected by the kanamycin A. The onset of hearing impairment generally occurred during the fourth week of kanamycin dosage although the earliest onset was towards the end of the second week. In most animals the hearing impairment progressed after kanamycin A was stopped and in one rat there was a latency between the end of drug dosage and onset of hearing impairment [2].
Clinical trial: Kanamycin A is an antibiotic used to treat severe bacterial infections and tuberculosis. It is not a first line treatment. Kanamycin A is recommended for short-term use only [3].
References:
[1] Misumi, M. and Tanaka, N. Mechanism of inhibition of translocation by kanamycin and viomycin: A comparative study with fusidic acid. Biochemical and Biophysical Research Communications 92(2), 647-654 (1980).
[2] Harpur ES, D'Arcy PF. The quantification of kanamycin ototoxicity in the rat using conditioned tone discrimination. J Pharm Pharmacol. 1975 Dec;27(12):907-13.
[3] https://en. wikipedia.org/wiki/Kanamycin_A
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 2.064 mL | 10.3199 mL | 20.6398 mL |
| 5 mM | 0.4128 mL | 2.064 mL | 4.128 mL |
| 10 mM | 0.2064 mL | 1.032 mL | 2.064 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。