Kanamycin Sulfate
(Synonyms: 硫酸卡那霉素; Kanamycin A sulfate) 目录号 : GC12269
An antibiotic used in molecular biology
Cas No.:25389-94-0
Sample solution is provided at 25 µL, 10mM.
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- Biological Activity: ≥750u/mg
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Kanamycin sulfate is an aminoglycoside bacteriocidal antibiotic which acts by binding to the bacterial 30S ribosomes.
Kanamycin sulfate at the concentration above 0.0025% has a significant inhibition on the growth of B. bifidum and has no influence on the other four probiotics at incubation 12 h or 24 h. The optimum selective concentration of kanamycin sulfate in MRS media is 0.005% for selective enumeration of B.bifidum[3]
The neurons damage of the DCN caused by kanamycin (500 mg/kg/day) is reversible and autophagy is upregulated in the neurotoxic course of kanamycin on DCN through JNK1-mediated phosphorylation of Bcl-2 pathway in rats. The serum BUN and Cr levels are both increased at the 1st day after the period of kanamycin administration. The neurons expressing LC3 are increased at 1, 7 and 14 days after kanamycin administration in comparison to the control group. Kanamycin treatment results in the increase of autophagy in a time-dependent manner[1]. Kanamycin sulfate (5 mg/kg) and sodium ampicillin (10 mg/kg) administered intramuscularly (i.m.) separately, and then together, to five pony mares, and the ampicillin concentration exceeds 5 mg/mL in inflamed synovial fluid for some 2.5 h after injection, and kanamycin sulfate concentration exceeds 2 mg/mL for 7 h in the pony[2].
Kanamycin sulfate是一种氨基糖苷类杀菌抗生素,通过结合到细菌30S核糖体发挥作用。 在培养12小时或24小时时,0.0025%以上的Kanamycin sulfate对B. bifidum的生长有显著的抑制作用,并且对其他四种益生菌没有影响。在MRS培养基中,Kanamycin sulfate的最佳选择浓度为0.005%,用于选择性枚举B.bifidum[3]。 大鼠的DCN神经元受到kanamycin (500mg/kg/day)损害是可逆的,并且在kanamycin对DCN的神经毒性过程中,自噬通过JNK1介导的Bcl-2途径磷酸化上调。在kanamycin给药后的第1天,血清BUN和Cr水平均增加。与对照组相比,kanamycin给药后1、7和14天表达LC3的神经元增加。kanamycin治疗导致自噬的时间依赖性增加[1]。将Kanamycin sulfate(5mg/kg)和氨苄青霉素钠(10mg/kg)分别通过肌肉注射(i.m.)给予五匹小马,然后一起给予,青霉素钠浓度在注射后的炎症滑液中超过5mg/mL,而Kanamycin sulfate的浓度在小马体内超过2mg/mL持续7小时[2]。
Reference:
[1]. Fan GR, et al. Reversible neurotoxicity of kanamycin on dorsal cochlear nucleus. Brain Res. 2013 Jan 17. pii: S0006-8993(13)00068-1.
[2]. Mohamed el SW, et al. Tongue actinomycetoma due to Actinomadura madurae: a rare clinical presentation. J Oral Maxillofac Surg. 2012 Nov;70(11):e622-4.
[3]. Guo WS, et al. Effect of Kanamycin Sulfate and Gentamicin on Growth of Probiotics. Advanced Materials Research,2011, 366, 490-493.
[4]. Firth EC, et al. Effect of induced synovial inflammation on pharmacokinetics and synovial concentration of sodium ampicillin and kanamycin sulfate after systemic administration in ponies. J Vet Pharmacol Ther. 1988 Mar;11(1):56-62.
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1 mg | 5 mg | 10 mg |
1 mM | 1.7165 mL | 8.5825 mL | 17.165 mL |
5 mM | 0.3433 mL | 1.7165 mL | 3.433 mL |
10 mM | 0.1717 mL | 0.8583 mL | 1.7165 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
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工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
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