Kanamycin Sulfate
(Synonyms: 硫酸卡那霉素; Kanamycin A sulfate) 目录号 : GC12269
An antibiotic used in molecular biology
Cas No.:25389-94-0
Sample solution is provided at 25 µL, 10mM.
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Kanamycin sulfate is an aminoglycoside bacteriocidal antibiotic which acts by binding to the bacterial 30S ribosomes.
Kanamycin sulfate at the concentration above 0.0025% has a significant inhibition on the growth of B. bifidum and has no influence on the other four probiotics at incubation 12 h or 24 h. The optimum selective concentration of kanamycin sulfate in MRS media is 0.005% for selective enumeration of B.bifidum[3]
The neurons damage of the DCN caused by kanamycin (500 mg/kg/day) is reversible and autophagy is upregulated in the neurotoxic course of kanamycin on DCN through JNK1-mediated phosphorylation of Bcl-2 pathway in rats. The serum BUN and Cr levels are both increased at the 1st day after the period of kanamycin administration. The neurons expressing LC3 are increased at 1, 7 and 14 days after kanamycin administration in comparison to the control group. Kanamycin treatment results in the increase of autophagy in a time-dependent manner[1]. Kanamycin sulfate (5 mg/kg) and sodium ampicillin (10 mg/kg) administered intramuscularly (i.m.) separately, and then together, to five pony mares, and the ampicillin concentration exceeds 5 mg/mL in inflamed synovial fluid for some 2.5 h after injection, and kanamycin sulfate concentration exceeds 2 mg/mL for 7 h in the pony[2].
Kanamycin sulfate是一种氨基糖苷类杀菌抗生素，通过结合到细菌30S核糖体发挥作用。 在培养12小时或24小时时，0.0025%以上的Kanamycin sulfate对B. bifidum的生长有显著的抑制作用，并且对其他四种益生菌没有影响。在MRS培养基中，Kanamycin sulfate的最佳选择浓度为0.005%，用于选择性枚举B.bifidum[3]。 大鼠的DCN神经元受到kanamycin (500mg/kg/day)损害是可逆的，并且在kanamycin对DCN的神经毒性过程中，自噬通过JNK1介导的Bcl-2途径磷酸化上调。在kanamycin给药后的第1天，血清BUN和Cr水平均增加。与对照组相比，kanamycin给药后1、7和14天表达LC3的神经元增加。kanamycin治疗导致自噬的时间依赖性增加[1]。将Kanamycin sulfate（5mg/kg）和氨苄青霉素钠（10mg/kg）分别通过肌肉注射（i.m.）给予五匹小马，然后一起给予，青霉素钠浓度在注射后的炎症滑液中超过5mg/mL，而Kanamycin sulfate的浓度在小马体内超过2mg/mL持续7小时[2]。
Reference:
[1]. Fan GR, et al. Reversible neurotoxicity of kanamycin on dorsal cochlear nucleus. Brain Res. 2013 Jan 17. pii: S0006-8993(13)00068-1.
[2]. Mohamed el SW, et al. Tongue actinomycetoma due to Actinomadura madurae: a rare clinical presentation. J Oral Maxillofac Surg. 2012 Nov;70(11):e622-4.
[3]. Guo WS, et al. Effect of Kanamycin Sulfate and Gentamicin on Growth of Probiotics. Advanced Materials Research,2011, 366, 490-493.
[4]. Firth EC, et al. Effect of induced synovial inflammation on pharmacokinetics and synovial concentration of sodium ampicillin and kanamycin sulfate after systemic administration in ponies. J Vet Pharmacol Ther. 1988 Mar;11(1):56-62.
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Animal experiment: |
Sixty-six male Sprague-Dawley rats (initial body weight 125-150 g, 5-6 weeks old) have free access to water and a regular diet, and are allowed 1 week of acclimation before the first treatment. The animals are divided randomly into one control group and seven experimental groups. Control group rats (n=10) are injected subcutaneously with an equal volume of vehicle (0.9% saline) for 10 days as those in the groups of kanamycin treatment, but without kanamycin. The experimental groups (n=56, 8 for each group: 1, 7, 14, 28, 56, 70 and 140 days after kanamycin administration, respectively) receive 500 mg of kanamycin sulfate/kg/day by subcutaneous injection for 10 days. The animal body weight is monitored every day and the injection dosage of kanamycin is adjusted accordingly. Auditory thresholds are tested by ABR. The tests are taken twice for each animal, first prior to the beginning of administration and then at different observing time points after kanamycin treatment. Details for the ABR measurement is described elsewhere. |
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References: [1]. Fan GR, et al. Reversible neurotoxicity of kanamycin on dorsal cochlear nucleus. Brain Res. 2013 Jan 17. pii: S0006-8993(13)00068-1. |
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| Cas No. | 25389-94-0 | SDF | |
| 别名 | 硫酸卡那霉素; Kanamycin A sulfate | ||
| 化学名 | 2-(aminomethyl)-6-[4,6-diamino-3-[4-amino-3,5-dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-2-hydroxycyclohexyl]oxyoxane-3,4,5-triol;sulfuric acid | ||
| Canonical SMILES | C1C(C(C(C(C1N)OC2C(C(C(C(O2)CN)O)O)O)O)OC3C(C(C(C(O3)CO)O)N)O)N.OS(=O)(=O)O | ||
| 分子式 | C18H36N4O11.H2SO4 | 分子量 | 582.58 |
| 溶解度 | ≥ 29.129mg/mL in Water | 储存条件 | Store at 2-8°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.7165 mL | 8.5825 mL | 17.165 mL |
| 5 mM | 0.3433 mL | 1.7165 mL | 3.433 mL |
| 10 mM | 0.1717 mL | 0.8583 mL | 1.7165 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Biological Activity: ≥750u/mg
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet