Kartogenin
(Synonyms: 2-([1,1-联苯]-4-基氨基甲酰)苯甲酸,KGN) 目录号 : GC15057
A potent inducer of chondrocyte differentiation
Cas No.:4727-31-5
Sample solution is provided at 25 µL, 10mM.
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Kartogenin is a potent inducer that promoting the differentiation of multipotent mesenchymal stem cells (MSCs) into chondrocytes with EC50 value of 100 nM [1].
MSCs are a kind of stem cell that can differentiate into different cell types including chondrocytes, osteoblasts, adipocytes and myocytes.
Kartogenin (KGN) potently induced the differentiation of hMSCs into chondrocytes in a dose-dependent way [1]. In preskeletal mesenchymal cells from mice, KGN stimulated the formations of cartilage nodule and synovial joint, boosted digit cartilaginous anlaga elongation and stimulated interdigit invagination, tendon maturation and interzone compaction. KGN increased the expression of TGFβ1, which stimulated cartilage nodule formation. Also, KGN increased the phosphorylation of Smads that mediated BMP and TGFβ signaling [2].
In the acute surgical model and the chronic joint injury model, kartogenin showed chondroprotective effects. Kartogenin disrupted the interaction of filamin A and the transcription factor core-binding factor β subunit (CBFβ) and regulated the CBFβ-RUNX1 transcriptional, which then induced chondrogenesis [1]. In the dermis of BALB/C mice, KGN induced synthesis of type-I collagen and activated Smad4/smad5 signaling pathway of the TGF-β [3].
References:
[1]. Johnson K, Zhu S, Tremblay MS, et al. A stem cell-based approach to cartilage repair. Science, 2012, 336(6082): 717-721.
[2]. Decker RS, Koyama E, Enomoto-Iwamoto M, et al. Mouse limb skeletal growth and synovial joint development are coordinately enhanced by Kartogenin. Dev Biol, 2014, 395(2): 255-267.
[3]. Wang J, Zhou J, Zhang N, et al. A heterocyclic molecule kartogenin induces collagen synthesis of human dermal fibroblasts by activating the smad4/smad5 pathway. Biochem Biophys Res Commun, 2014, 450(1): 568-574.
Cell experiment: | Rabbit BMSCs or PTSCs are treated with various concentrations (1 nM to 5 μM) of kartogenin. The medium is changed every 3 days and after 2 weeks, cell proliferation is measured by population doubling time[1]. |
Animal experiment: | Rats: Then rats are divided into two groups based on the injections received: six rats are given 10 µL saline injections in each wound (wound-only group) and six rats receive 10 µL of 100 µM kartogenin solution each in the wounded areas (wound+kartogenin group). The injections are given immediately after wounding and repeated on days 2, 4, 7 and 12[1]. |
References: [1]. Zhang J, et al. Kartogenin induces cartilage-like tissue formation in tendon-bone junction. Bone Res. 2014;2. pii: 14008. | |
| Cas No. | 4727-31-5 | SDF | |
| 别名 | 2-([1,1-联苯]-4-基氨基甲酰)苯甲酸,KGN | ||
| 化学名 | 2-[(4-phenylphenyl)carbamoyl]benzoic acid | ||
| Canonical SMILES | O=C(C1=CC=CC=C1C(O)=O)NC2=CC=C(C=C2)C3=CC=CC=C3 | ||
| 分子式 | C20H15NO3 | 分子量 | 317.34 |
| 溶解度 | ≥ 10.6mg/mL in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.1512 mL | 15.756 mL | 31.5119 mL |
| 5 mM | 0.6302 mL | 3.1512 mL | 6.3024 mL |
| 10 mM | 0.3151 mL | 1.5756 mL | 3.1512 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet