Kisspeptin-10 Trifluoroacetate
目录号 : GC34403
Kisspeptin-10Trifluoroacetate是Kisspeptin-10的三氟乙酸盐形式。Kisspeptin-10是具有全部生物活性的Kisspeptin最短多肽段，它是一种有效的GPR54内源性配体。
Sample solution is provided at 25 µL, 10mM.
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Kisspeptin-10 Trifluoroacetate is the trifluoroacetate salt form of Kisspeptin-10. Kisspeptin-10, the minimal kisspeptin sequence with biological activity, is a potent endogenous ligand for GPR54.
Kisspeptin-10 is the minimal kisspeptin sequence with full intrinsic bioactivity. Kisspeptins are a family of peptides encoded by the Kiss1 gene and are the natural ligands of the orphan g protein-coupled receptor GPR54[1]. Kisspeptins act as the principal positive regulator of the reproductive axis by directly stimulating gonadotropin-releasing hormone (GnRH) neuron activity. Kisspeptin-10 also has a direct stimulating effect on luteinizing hormone (LH) secretion in bovine anterior pituitary (AP) cells[2]. Different concentrations of Kisspeptin-10 have distinct effects on migration and proliferation of endothelial cells. Migration and proliferation of endothelial cells are increased at lower concentration of Kisspeptin-10 specially at 100 nM while higher concentration reduced both migration and proliferation[3].
Intravenous infusion of kisspeptin-10 (7.5, 35, and 100 nM) induces a dose-dependent increase in LH secretion. The stimulatory effect of kisspeptin-10 (100 n nM) on LH secretion is blocked by the GnRH antagonist cetrorelix, precluding a singular action on gonadotropes[4]. Kisspeptin-10 inhibits angiogenesis in vivo. Kp-10 inhibits tumor growth in SCID mice xenografted with human prostate cancer cells (PC-3) through inhibiting tumor angiogenesis[5].
[1]. Kotani M, et al. The metastasis suppressor gene KiSS-1 encodes kisspeptins, the natural ligands of the orphan G protein-coupled receptor GPR54. The metastasis suppressor gene KiSS-1 encodes kisspeptins, the natural ligands of the orphan G protein-coupled receptor GPR54. [2]. Kadokawa H, et al. Kisspeptin-10 stimulates the secretion of growth hormone and prolactin directly from cultured bovine anterior pituitary cells. Anim Reprod Sci. 2008 May;105(3-4):404-8. [3]. Golzar F, et al. The effects of kisspeptin-10 on migration and proliferation of endothelial cell. Adv Biomed Res. 2015 Feb 11;4:41. [4]. Kinsey-Jones JS, et al. Effects of kisspeptin-10 on the electrophysiological manifestation of gonadotropin-releasing hormone pulse generator activity in the female rat. Endocrinology. 2008 Mar;149(3):1004-8. [5]. Cho SG, et al. Kisspeptin-10, a KISS1-Derived Decapeptide, Inhibits Tumor Angiogenesis by Suppressing Sp1-Mediated VEGF Expression and FAK/Rho GTPase Activation. Cancer Res. 2009 Sep 1;69(17):7062-70.
| Cas No. | SDF | ||
| Canonical SMILES | Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH2 | ||
| 分子式 | C63H83N17O14.C2HF3O2 | 分子量 | 1416.46 |
| 溶解度 | Water : 4 mg/mL (2.82 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.706 mL | 3.5299 mL | 7.0599 mL |
| 5 mM | 0.1412 mL | 0.706 mL | 1.412 mL |
| 10 mM | 0.0706 mL | 0.353 mL | 0.706 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet